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Achim Knappik
Researcher at MorphoSys
Publications - 43
Citations - 3675
Achim Knappik is an academic researcher from MorphoSys. The author has contributed to research in topics: Monoclonal antibody & Phage display. The author has an hindex of 17, co-authored 42 publications receiving 3542 citations. Previous affiliations of Achim Knappik include University of Ulm & University of Zurich.
Papers
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Journal ArticleDOI
Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides.
Achim Knappik,Liming Ge,Annemarie Honegger,Peter Pack,Melanie Fischer,Günter Wellnhofer,Adolf Hoess,Joachim Wölle,Andreas Plückthun,Bernhard Virnekäs +9 more
TL;DR: The small number of 49 master genes will allow future improvements to be incorporated quickly, and the separation of the frameworks may help in analyzing why nature has evolved these distinct subfamilies of antibody germline genes.
Patent
Protein/(poly)peptide libraries
TL;DR: In this article, a method for synthesizing a library of human-derived antibody genes by the use of synthetic consensus sequences which cover the structural repertoire of antibodies encoded in the human genome was proposed.
Journal ArticleDOI
Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display
TL;DR: This work has mimicked the process of antibody generation and affinity maturation with a synthetic library in a cell-free system in just a few days, obtaining molecules with higher affinities than most natural antibodies.
Journal ArticleDOI
Engineered turns of a recombinant antibody improve its in vivo folding
Achim Knappik,Andreas Plückthun +1 more
TL;DR: This analysis shows that it is possible to engineer improved frameworks for semi-synthetic antibody libraries which may be important in maintaining library diversity and that limitations in recombinant protein expression can be overcome by single amino acid substitutions.
Journal ArticleDOI
High-throughput generation and engineering of recombinant human antibodies.
Barbara Krebs,Robert Rauchenberger,Silke Reiffert,Christine Rothe,Michael Tesar,Elisabeth Thomassen,Manqiu Cao,Torsten Dreier,David Fischer,Adolf Höß,Landon Inge,Achim Knappik,Matthias Marget,Peter Pack,Xian-Qin Meng,Robert Schier,Peter Söhlemann,Jill Winter,Joachim Wölle,Titus Kretzschmar +19 more
TL;DR: It is demonstrated that since HuCAL-scFv antibodies are expressed in high levels in Escherichia coli, automated panning and screening in miniaturised settings (96- and 384-well format) have now become feasible and HuCal is a very convenient source of human antibodies for various applications.