Showing papers in "Journal of Immunological Methods in 2001"

TL;DR: The immuno-magnetic isolation and analysis ofExosomes is a versatile and rapid tool for the analysis of APC exosomes, and may prove a valuable tool forThe study of exosome biology.

TL;DR: Over overlapping 15 amino acid peptide mixes may facilitate the analysis of antigen-specific CD4+ and CD8+ T-cell responses by cytokine flow cytometry, using clinical specimens that include shipped blood or cryopreserved PBMC.

TL;DR: This review describes the design and expression of diabodies, triabodies and tetrabodies using examples of scFv molecules that target viruses and cancer and highlights a number of cancer-targeting scFV multimers that have recently successfully undergone pre-clinical trials for in vivo stability and efficacy.

TL;DR: The newly developed Becton Dickinson Cytometric Bead Array (CBA), which combines the principles of the "sandwich" immunoassay with the capability of flow cytometry for simultaneous measurement of the characteristics of multiple particles, allows determination of six human cytokine concentrations simultaneously in a single tear sample.

TL;DR: This chapter will focus on current strategies used to generate protein and antibody arrays and their current applications in biological research, medicine and diagnostics.

TL;DR: A robust technology for the creation of bispecific IgG has recently been developed that virtually precludes IgG contaminants, as reviewed here, and this technology is anticipated to spur the clinical development of bisPespecific IgG and other bifunctional Fc-containing molecules such as antibody/immunoadhesin hybrids and bispespecific immunoadhesins.

TL;DR: It is demonstrated that since HuCAL-scFv antibodies are expressed in high levels in Escherichia coli, automated panning and screening in miniaturised settings (96- and 384-well format) have now become feasible and HuCal is a very convenient source of human antibodies for various applications.

TL;DR: An enzyme-linked immunosorbent assay (ELISA) system has been developed that uses glutathione crosslinked to casein as capture protein to bind recombinant protein antigens fused to N-terminal glutathion S-transferase (GST).

TL;DR: The specific C4b-depositing capacity of the MBL pathway was determined by incubating serum diluted in buffer containing 1 M NaCl in mannan-coated microtiter wells before the addition of purified C4.

TL;DR: Homing of T cells into the spleen was observed by a decrease in MRI signal intensity within 1 h after the transfer, which remained decreased for 2-24 h after transfer, and the biodistribution of FITC-CLIO-Tat loaded T cells can be monitored in vivo over time by MRI.

TL;DR: The development and characterization of the CyQUANT cell proliferation assay is described, a highly sensitive, fluorescence-based microplate assay for determining numbers of cultured cells that correlated linearly with cell number over a large range using a wide variety of cell types.

TL;DR: A new flow cytometry method, developed in the context of CD95-mediated cell death, which allows the precise quantitation of cell-mediated cytotoxicity and the detection of intracellular events involved in the cytotoxic process and facilitates the analysis of some events of the apoptotic pathway.

TL;DR: A standard protocol for preparation, maintenance and quality control of human endothelial cells is described, which is physiologically more relevant than many established cell lines.

TL;DR: An outline of the original method, several studies achieved by using the method as a major strategic tool, technological difficulties and intrinsic problems that emerged, and improvements and modifications of the method to cope with these drawbacks are presented.

TL;DR: In animals bearing tumors, antibody-cytokine fusion proteins are able to target the tumor and to elicit a significant anti-tumor response that in some cases results in a complete elimination of the tumor.

TL;DR: Experiments demonstrate that multiple cytokines and antibodies can be simultaneously detected using this new approach, and the concept should be able to be extended to develop a high-throughput protein array system.

TL;DR: The scFv-Fc fusion is capable of mediating antibody-dependent cellular cytotoxicity against tumor target cells using human peripheral blood mononuclear cells as effectors and the Fc domain is a convenient, robust affinity handle for purification and immunochemical applications, eliminating the need for proteolytically sensitive epitope and/or affinity tags on the scFV.

TL;DR: A novel flow cytometric assay which addresses and improves upon the problems currently encountered with the 51Cr release assay and will be of value to further investigate mechanisms of cytolysis by effector cell populations.

TL;DR: These studies establish the conditions for the optimal transfection of effector lymphocytes to redirect them against a variety of tumor targets.

TL;DR: The specificity of the QDs-labeled immunoglobulin (IgG) was tested by an experiment using goat IgG and human IgG samples and the result was consistent with the binding specificity in a sandwich-type assay.

TL;DR: The use of the chicken DT40 B cell line is increasing in popularity due to the ease with which it can be manipulated genetically, and advantage has been taken of this to investigate such diverse fields as B cell antigen receptor (BCR) signaling, cell cycle regulation, gene conversion and apoptosis.

TL;DR: This review presents an overview of strategies that employ computer algorithms for the selection of candidate peptides from defined proteins and subsequent verification of their in vivo relevance by experimental approaches.

TL;DR: The results demonstrate how phage antibodies binding native cell surface receptors can be directly selected on overexpressing cell lines or transfected cells.

TL;DR: The application of ribosome display and selection in conjunction with variable domain (CTLA-4) libraries as the first step towards this objective are discussed and affinity maturation strategies for in vitro ribosomes display systems are reviewed.

TL;DR: It is concluded that the Alamar Blue-assay combined with a hollow-fiber bioreactor offers distinct advantages for the non-invasive monitoring of cell viability and proliferation in a closed system.

TL;DR: The data demonstrate the need for data on the impact of ex vivo variation in order to extract reliable and consistent information, particularly when cytokine mRNA expression data from healthy blood donors are included in clinical studies.

TL;DR: During a 7-day co-culture of peripheral blood mononuclear cells and KRN7000-loaded mature monocyte derived DC (moDC) in the presence of interleukin-7 (IL-7) and IL-15, it is observed up to 76-fold expansion of Valpha24(+)Vbeta11(+)-T cells.

TL;DR: This method was developed for the study of erythropoietin (EPO) in concentrated urine since a strong non-specific binding of biotinylated secondary antibodies to some urinary proteins had been observed using classical IB protocols.

TL;DR: A protocol has been outlined to develop sandwich ELISAs for cytokines using commercial antibodies that yield low background, detect a wide range of concentrations, and are suitable for use in the research setting.

TL;DR: A class of DGE technologies collectively referred to as 'open' architecture systems, distinct from the 'closed' DGE Technologies, in that no pre-existing biological or sequence information is necessary and they are applicable to any species are reviewed.