Achim Wach

TL;DR: A new set of plasmids that serve as templates for the PCR synthesis of fragments that allow a variety of gene modifications that should further facilitate the rapid analysis of gene function in S. cerevisiae.

TL;DR: A dominant resistance module, for selection of S. cerevisiae transformants, which entirely consists of heterologous DNA is constructed and tested, and some kanMX modules are flanked by 470 bp direct repeats, promoting in vivo excision with frequencies of 10–3–10–4.

TL;DR: A straightforward PCR‐based approach to the deletion, tagging, and overexpression of genes in their normal chromosomal locations in the fission yeast Schizosaccharomyces pombe, and a series of plasmids containing the kanMX6 module, which allows selection of G418‐resistant cells and thus provides a new heterologous marker for use in S. pom be.

TL;DR: A PCR‐method for fast production of disruption cassettes is introduced, that allows the addition of long flanking homology regions of several hundred base pairs (LFH‐PCR) to a marker module, which will help to apply PCR‐mediated gene manipulations to strains with decreased transformability and/or unpredictable sequence deviations.

TL;DR: GFP reporters consist of wild‐type GFP or GFP‐S65T coding sequences, lacking the ATG, fused to the S. cerevisiae ADH1 terminator and PCR‐synthesized 2·4 kb‐long double modules flanked by 40 bp‐long guide sequences were successfully targeted to the carboxy‐terminus of a number of S. Cerevisiae genes.