A
Achim Wach
Researcher at University of Basel
Publications - 14
Citations - 12017
Achim Wach is an academic researcher from University of Basel. The author has contributed to research in topics: Gene & Saccharomyces cerevisiae. The author has an hindex of 12, co-authored 13 publications receiving 11422 citations. Previous affiliations of Achim Wach include University of North Carolina at Chapel Hill.
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Journal ArticleDOI
Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
Mark S. Longtine,Amos Mckenzie,Douglas J. Demarini,Nirav Shah,Achim Wach,Arndt Brachat,Peter Philippsen,John R. Pringle +7 more
TL;DR: A new set of plasmids that serve as templates for the PCR synthesis of fragments that allow a variety of gene modifications that should further facilitate the rapid analysis of gene function in S. cerevisiae.
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New heterologous modules for classical or PCR‐based gene disruptions in Saccharomyces cerevisiae
TL;DR: A dominant resistance module, for selection of S. cerevisiae transformants, which entirely consists of heterologous DNA is constructed and tested, and some kanMX modules are flanked by 470 bp direct repeats, promoting in vivo excision with frequencies of 10–3–10–4.
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Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe
Jürg Bähler,Jian-Qiu Wu,Mark S. Longtine,Nirav Shah,Amos Mckenzie,Alexander B. Steever,Achim Wach,Peter Philippsen,John R. Pringle +8 more
TL;DR: A straightforward PCR‐based approach to the deletion, tagging, and overexpression of genes in their normal chromosomal locations in the fission yeast Schizosaccharomyces pombe, and a series of plasmids containing the kanMX6 module, which allows selection of G418‐resistant cells and thus provides a new heterologous marker for use in S. pom be.
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PCR‐synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiae
TL;DR: A PCR‐method for fast production of disruption cassettes is introduced, that allows the addition of long flanking homology regions of several hundred base pairs (LFH‐PCR) to a marker module, which will help to apply PCR‐mediated gene manipulations to strains with decreased transformability and/or unpredictable sequence deviations.
Journal ArticleDOI
Heterologous HIS3 marker and GFP reporter modules for PCR-targeting in Saccharomyces cerevisiae.
TL;DR: GFP reporters consist of wild‐type GFP or GFP‐S65T coding sequences, lacking the ATG, fused to the S. cerevisiae ADH1 terminator and PCR‐synthesized 2·4 kb‐long double modules flanked by 40 bp‐long guide sequences were successfully targeted to the carboxy‐terminus of a number of S. Cerevisiae genes.