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Aihua Zhang
Researcher at New England Biolabs
Publications - 10
Citations - 352
Aihua Zhang is an academic researcher from New England Biolabs. The author has contributed to research in topics: SNAP-tag & Fluorescence microscope. The author has an hindex of 5, co-authored 10 publications receiving 315 citations.
Papers
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Journal ArticleDOI
Development of SNAP-tag fluorogenic probes for wash-free fluorescence imaging
Xiaoli Sun,Aihua Zhang,Brenda Baker,Luo Sun,Angela Howard,John Buswell,Damien Maurel,Anastasiya Masharina,Kai Johnsson,Christopher J. Noren,Ming-Qun Xu,Ivan R. Corrêa +11 more
TL;DR: A fast‐labeling variant of SNAP‐tag, termed SNAPf, is characterized, which displays up to a tenfold increase in its reactivity towards benzylguanine substrates and enables highly sensitive spatiotemporal investigation of protein dynamics in living cells.
Journal ArticleDOI
Substrates for Improved Live-Cell Fluorescence Labeling of SNAP-tag
Ivan R. Corrêa,Brenda Baker,Aihua Zhang,Luo Sun,Christopher R. Provost,Grazvydas Lukinavicius,Luc Reymond,Kai Johnsson,Ming-Qun Xu +8 more
TL;DR: These new SNAP-tag substrates based on pure 6-regioisomers can significantly improve fluorescence labeling in live cells and should become powerful tools for bioimaging applications.
Journal ArticleDOI
Near-Infrared Fluorescence Imaging of Mammalian Cells and Xenograft Tumors with SNAP-Tag
Haibiao Gong,Joy L. Kovar,Brenda Baker,Aihua Zhang,Lael Cheung,Daniel R. Draney,Ivan R. Corrêa,Ming-Qun Xu,D. Michael Olive +8 more
TL;DR: In vivo imaging study showed that BG-800 could be used to visualize xenograph tumors expressing SNAPf-ADRβ2 expressing tumor, and quenched substrates that only fluoresce upon reaction with SNAP-tag were synthesized and characterized.
Book ChapterDOI
Site-specific protein labeling by intein-mediated protein ligation.
Inca Ghosh,Nancy Considine,Elissa Maunus,Luo Sun,Aihua Zhang,John Buswell,Thomas C. Evans,Ming-Qun Xu +7 more
TL;DR: A procedure to conduct isolation and labeling of recombinant proteins expressed in E. coli using synthetic short peptides possessing a fluorescent moiety is presented.
Journal ArticleDOI
Solid-phase enzyme catalysis of DNA end repair and 3' A-tailing reduces GC-bias in next-generation sequencing of human genomic DNA.
Aihua Zhang,Shaohua Li,Lynne Apone,Xiaoli Sun,Lixin Chen,Laurence Ettwiller,Bradley W. Langhorst,Christopher J. Noren,Ming-Qun Xu +8 more
TL;DR: Evidence is found that a major source of bias is the inefficient processing of AT-rich DNA in end repair and 3′ A-tailing, causing under-representation of extremely At-rich regions of the human genome with existing amplification-free DNA library preparation methods.