Albert M. Cheh

TL;DR: Although the solution chemistry of diol epoxides is now fairly well understood, a great deal remains to be elucidated regarding their reaction in the presence of DNA, and detailed conformational analysis of adducted DNA should prove to be extremely valuable in developing mechanistic models for the enzymatic processing of chemically altered DNA.

TL;DR: In this paper, the structure of the principal covalent adducts formed from DNA upon reaction in vitro with the four optically active 3,4-diol 1,2-epoxides of benz[a]anthracene have been elucidated at the nucleoside level.

TL;DR: The potent environmental carcinogen benzo[a]pyrene, following enzymatic activation to enantiomeric pairs of bay-region 7,8-diol 9, 10-epoxides, reacts with DNA to form covalent adducts predominately at the exocyclic amino groups of purines that are incorporated into plasmids by primer extension, followed by purification of the covalently closed circular constructs.

TL;DR: The restricted ability of deoxyribonuclease I/snake venom phosphodiesterase digestion to liberate deoxyadenosine (dA) nucleotide adducts of polycyclic aromatic hydrocarbons from DNA is observed and two digestion procedures exhibit systematic and mostly opposite stereoselectivity in the pattern of which dAAdducts are resistant to digestion.