A
Alfredo F. Braña
Researcher at University of Oviedo
Publications - 154
Citations - 6458
Alfredo F. Braña is an academic researcher from University of Oviedo. The author has contributed to research in topics: Gene cluster & Streptomyces. The author has an hindex of 49, co-authored 152 publications receiving 5988 citations. Previous affiliations of Alfredo F. Braña include Massachusetts Institute of Technology & International Sleep Products Association.
Papers
More filters
Journal ArticleDOI
Combinatorial biosynthesis of antitumor indolocarbazole compounds
Cesar Sanchez,Lili Zhu,Alfredo F. Braña,Aaroa Pérez Salas,Jürgen Rohr,Carmen Méndez,José A. Salas +6 more
TL;DR: A biological process based on combinatorial biosynthesis for the production of indolocarbazole compounds (or their precursors) in engineered microorganisms as a complementary approach to chemical synthesis is reported.
Journal ArticleDOI
The Biosynthetic Gene Cluster for the Antitumor Rebeccamycin: Characterization and Generation of Indolocarbazole Derivatives
TL;DR: The cloned genes should help to elucidate the molecular basis for indolocarbazole biosynthesis and set the stage for the generation of novel indolo-rebeccamycin analogues by genetic engineering.
Journal ArticleDOI
Identification of Two Genes from Streptomyces argillaceus Encoding Glycosyltransferases Involved in Transfer of a Disaccharide during Biosynthesis of the Antitumor Drug Mithramycin
Ernestina Fernández,Ulrike Weißbach,César Sánchez Reillo,Alfredo F. Braña,Carmen Méndez,Jürgen Rohr,Jürgen Rohr,José A. Salas +7 more
TL;DR: It is proposed that the glycosyltransferases encoded by mtmGI and mtmGII are responsible for forming and transferring the disaccharide during mithramycin biosynthesis.
Journal ArticleDOI
Activation and identification of five clusters for secondary metabolites in Streptomyces albus J1074
Carlos Olano,Ignacio García,Aranzazu Valdés González,Miriam Rodríguez,Daniel Rozas,Julio M. Rubio,Marina Sánchez-Hidalgo,Alfredo F. Braña,Carmen Méndez,José A. Salas +9 more
TL;DR: A cluster for glycosylated compounds (paulomycins) was also identified by comparison of the high‐performance liquid chromatography profiles of the wild‐type strain with that of a mutant in which two key enzymes of the cluster were simultaneously deleted.
Journal ArticleDOI
Deciphering the biosynthesis pathway of the antitumor thiocoraline from a marine actinomycete and its expression in two Streptomyces species
Felipe Lombó,Ana Velasco,Angelina Castro,Fernando de la Calle,Alfredo F. Braña,José María Sánchez-Puelles,Carmen Méndez,José A. Salas +7 more
TL;DR: Surprisingly, the identified gene cluster contains more NRPS modules than expected on the basis of the number of amino acids of thiocoraline, and it is proposed that two other NRPSs, TioY and TioZ, could be responsible for the biosynthesis of a small peptide molecule which could be involved in regulation of the biosynthetic of thicoraline in Micromonospora sp.