Ali Farrokhi

TL;DR: The differentiation of hESCs into hepatocyte-like cells within 3D collagen scaffolds containing exogenous growth factors gives rise to cells displaying morphological features, gene expression patterns and metabolic activities characteristic of hepatocytes and may provide a source of differentiated cells for treatment of liver diseases.

TL;DR: The derivation and characterization of five hESC lines on mouse embryonic fibroblast cells is presented, and the pluripotency of the cell line was confirmed by spontaneous differentiation under in vitro conditions.

TL;DR: A novel establishment and maintenance culture technique that uses human dermal fibroblasts to generate hiPSCs by introducing four factors, Klf4, Oct4, Sox2, and c-Myc under serum- and feeder-independent conditions is described.

TL;DR: It is shown that FGF4 and TGFβ signaling pathway inhibitors, designated R2i, not only provide the ground state pluripotency in production and maintenance of naïve ES cells from blastocysts of different mouse strains, but also maintain ES cells with higher genomic integrity following long-term cultivation compared with the chemical inhibition of the FGF 4 and GSK3 pathways.

TL;DR: Significant insulin concentrations in protocol 1 were produced when glucose was added to the medium, in comparison with protocol 2, and improvements of the current method are required to generate a sufficient source of true beta‐cells for the treatment of diabetes mellitus.