Showing papers by "Allan Munck published in 1980"
Journal Article•
TL;DR: Observations indicate that formation of new glucocorticoid receptors near the S phase may be a general phenomenon in proliferating cells, and propose that this increase in glucocortex receptors during the cell cycle may explain the increase in glucose receptors in mitogen-stimulated lymphocytes.
Abstract: To study variations in glucocorticoid receptor levels during the cell cycle, we have separated mitogen-stimulated human peripheral lymphocytes and rat lymph node cells by unit gravity sedimentation and measured glucocorticoid binding in the resultant fractions. By morphologic criteria and thymidine incorporation, the fractions were separated into populations of G0 and G1 phase and S and post-S phase cells. A 2- to 3-fold increase in glucocorticoid receptor sites per cell, for cells in the S and post-S phase over those in G0 and G1, was observed with both nonstimulated rat lymph node cell suspensions and concanavalin A-stimulated human peripheral lymphocytes. These observations together with those from other studies indicate that formation of new glucocorticoid receptors near the S phase may be a general phenomenon in proliferating cells. We propose that this increase in glucocorticoid receptors during the cell cycle may explain the increase in glucocorticoid receptors in mitogen-stimulated lymphocytes.
56 citations
TL;DR: Complex Ia may be a third normal form of extranuclear glucocorticoid-rcceptor complex, but the possibility remains that it is an artifact formed after cells are broken, in agreement with earlier results.
47 citations
TL;DR: It is proposed that an important component in glucocorticoid-induced immunosuppression may be a reduction in Fc receptors, which play important roles in phagocytosis and other aspects of immune responses.
43 citations
Journal Article•
TL;DR: Despite a 50% increase in the number of glucocorticoid receptor sites per cell, the cells from the homolateral and controlateral lymph nodes were equally sensitive to the inhibitory effects of dexamethasone, as determined by measurements of the incorporation of radiolabeled precursors of protein, RNA, and DNA, or measurements of in vitro cell survival.
Abstract: Recently a 2- to 3-fold increase in the number of glucocorticoid receptors in human peripheral lymphocytes has been noted after in vitro mitogen stimulation. Here, we extend these observations to in vivo immunization. After unilateral immunization of adrenalectomized male rats, a 50% increase in glucocorticoid receptor sites per cell, determined by binding of dexamethasone, was observed in cell suspensions of homolateral lymph nodes over those from the contralateral nonimmunized side of the same animal. The association constant for dexamethasone was similar in both groups, as was the stereospecificity for various steroids, the time course of cytoplasmic and nuclear association, and cytoplasmic-to-nuclear translocation. Despite a 50% increase in the number of glucocorticoid receptor sites per cell, the cells from the homolateral and controlateral lymph nodes were equally sensitive to the inhibitory effects of dexamethasone, as determined by measurements of the incorporation of radiolabeled precursors of protein, RNA, and DNA, or measurements of in vitro cell survival.
39 citations
TL;DR: In this article, the authors used differential salt extraction of nuclei from cells incubated with glucocorticoids under various conditions to further characterize the nature of the nuclear receptor-receptor interaction.
24 citations
TL;DR: Results suggest glucocorticoids also produce changes in methionine incorporation and phosphorylation of proteins in the cytosol fraction, and may reflect a physiologically important inhibitory effect on lipid synthesis with long-term consequences for membrane structure and function.
15 citations
TL;DR: This study analyzes the sensitivity of nuclear bound glucocorticoid receptors to solubilization from nuclei by DNAase I and DNAase II and extracts of nuclear dexamethasone-receptor chromatograph as two peaks of radioactivity.
11 citations