Alon Greenbaum

TL;DR: This Q&A delineates recent advances and practical challenges associated with these two techniques when applied body-wide in tissue clearing and optogenetics.

TL;DR: In this paper, the authors demonstrate a high-throughput charged particle analysis platform based on lensfree on-chip microscopy for rapid ion track analysis using allyl diglycol carbonate, i.e., CR-39 plastic polymer as the sensing medium.

TL;DR: In this paper , the illumination beam angular properties are controlled by two galvanometer scanners, while a deformable mirror is positioned in the detection path to correct for optical aberrations.

TL;DR: In this paper , the porcine cochlea was analyzed in 3D using tissue clearing and light-sheet microscopy, and the resulting 3D images can be employed to compare cochelleae across different ages and conditions, investigate the ontogeny of cochlear cytoarchitecture, and produce quantitative expression maps of LGR5, a marker of co-lear progenitors in mice.

TL;DR: Light-guided sectioning (LiGS) as discussed by the authors preserves the tissue with its optical implant in place and allows staining and clearing of a volume up to 500μm in depth, which could help increase reproducibility through identification of fiber-to-target localization and molecular profiling.