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Alyssa J. Miller
Researcher at University of Michigan
Publications - 31
Citations - 1725
Alyssa J. Miller is an academic researcher from University of Michigan. The author has contributed to research in topics: Organoid & Progenitor cell. The author has an hindex of 13, co-authored 26 publications receiving 1098 citations. Previous affiliations of Alyssa J. Miller include Harvard University.
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Journal ArticleDOI
Synthetic hydrogels for human intestinal organoid generation and colonic wound repair
Ricardo Cruz-Acuña,Miguel Quiros,Attila Farkas,Priya H. Dedhia,Sha Huang,Dorothee Siuda,Vicky Garcia-Hernandez,Alyssa J. Miller,Jason R. Spence,Asma Nusrat,Andrés J. García +10 more
TL;DR: A fully defined, synthetic hydrogel based on a four-armed, maleimide-terminated poly(ethylene glycol) macromer that supports robust and highly reproducible in vitro growth and expansion of HIOs, such that three-dimensional structures are never embedded in tumour-derived ECM is developed.
Journal ArticleDOI
Generation of lung organoids from human pluripotent stem cells in vitro.
Alyssa J. Miller,Briana R. Dye,Daysha Ferrer-Torres,David R. Hill,Arend W. Overeem,Lonnie D. Shea,Jason R. Spence +6 more
TL;DR: A protocol that recapitulates several of these milestones in order to differentiate human pluripotent stem cells (hPSCs) into ventral–anterior foregut spheroids and further into two distinct types of organoids: human lung organoids and bud tip progenitor organoids.
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In Vitro Induction and In Vivo Engraftment of Lung Bud Tip Progenitor Cells Derived from Human Pluripotent Stem Cells
Alyssa J. Miller,David R. Hill,Melinda S. Nagy,Yoshiro Aoki,Briana R. Dye,Alana M. Chin,Sha Huang,Felix Zhu,Eric S. White,Vibha N. Lama,Jason R. Spence +10 more
TL;DR: Molecular and cellular comparisons revealed that hPSC-derived bud tip-like cells are highly similar to native lung bud tip progenitors.
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A bioengineered niche promotes in vivo engraftment and maturation of pluripotent stem cell derived human lung organoids
Briana R. Dye,Priya H. Dedhia,Alyssa J. Miller,Melinda S. Nagy,Eric S. White,Lonnie D. Shea,Jason R. Spence +6 more
TL;DR: It is shown that in vivo transplanted hPSC-derived human lung organoids had improved cellular differentiation of secretory lineages that is reflective of differences between fetal and adult tissue, resulting in airway-like structures that were remarkably similar to the native adult human lung.
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In vitro models to study human lung development, disease and homeostasis
Alyssa J. Miller,Jason R. Spence +1 more
TL;DR: Current advances in generating in vitro human lung models using primary human tissue, cell lines, and human pluripotent stem cell derived lung tissue are discussed, and crucial next steps in the field are discussed.