Amelia Au-Yeung

TL;DR: Large-scale single-cell sequencing of RNA and T cell receptors in samples from patients with cancer shows clonotypic expansion of effector-like T cells not only in tumour tissue but also in normal adjacent tissues and peripheral blood, which associates with clinical response to cancer immunotherapy.

TL;DR: In this article , Chen et al. studied the mechanisms whereby PD-1 and/or TIGIT blockade modulate anti-tumor CD8+ T cells and found that the effectiveness of PD-L1 or anti-TIGIT inhibition in preclinical tumor models was reduced in the absence of CD226.

TL;DR: How signal overlap can complicate mass cytometry data interpretation is visualize and how the rational distribution of interference can greatly improve panel design and data quality is demonstrated.

TL;DR: In this article, the presence of CD103+ CD8+ TRM cells, quantified by tracking intratumoral CD103 expression, can predict treatment outcome, suggesting that patients who respond to PD-1/PD-L1 blockade are those who exhibit an ongoing antitumor T-cell response.

TL;DR: These studies highlight the consistency between t-SNE and conventional hand-gating in stratifying general immune cell lineages while demonstrating that particular cell subsets defined by conventional manual gating may be intermingled in t- SNE space.