Showing papers by "Ana J. García-Sáez published in 2011"
TL;DR: In this paper, the authors investigated the role of plasma membrane organization for equinatoxin action and found that the reorganization of the plasminar membrane into stabilized lipid rafts is part of the killing strategy.
53 citations
TL;DR: The last developments to quantify protein/protein interactions in lipid membranes are reviewed and the use of fluorescence-correlation-spectroscopy approaches for that purpose is focused on.
Abstract: The characterization of interactions between membrane proteins as they take place within the lipid bilayer poses a technical challenge, which is currently very difficult and, in many cases, impossible to overcome. The recent development of a method based in the combination two-color fluorescence cross-correlation spectroscopy with scanning of the focal volume allows the detection and quantification of interactions between biomolecules inserted in biological membranes. This powerful strategy has allowed the quantitative analysis of diverse systems, such as the association between proteins of the Bcl-2 family involved in apoptosis regulation or the binding between a growth factor and its receptor during signaling. Here, we review the last developments to quantify protein/protein interactions in lipid membranes and focus on the use of fluorescence-correlation-spectroscopy approaches for that purpose.
9 citations
TL;DR: It is speculated that confinement into envelope fluid nanodomains might boost the distinct capacities of HIV MPER hydrophobic modules for inducing membrane defects during fusion.
8 citations