Showing papers by "Ana Maria Oliveira Battastini published in 2008"
TL;DR: Results suggest that the participation of ecto-5′-NT/CD73 in cell proliferation is dependent upon the enzyme’s production of adenosine, a proliferative factor, and removal of AMP, a toxic molecule for gliomas.
Abstract: Malignant gliomas are the most common and devastating primary tumors in the brain and, despite treatment, patients with these tumors have a poor prognosis. The participation of ecto-5′-NT/CD73 per se as a proliferative factor, being involved in the control of cell growth, differentiation, invasion, migration and metastasis processes has been previously proposed. In the present study, we evaluated the activity and functions of ecto-5′-NT/CD73 during the proliferation process of rat C6 and human U138MG glioma cell lines. Increasing confluences and culture times led to an increase in ecto-5′-NT/CD73 activity in both C6 and U138MG glioma cells. RT-PCR analysis and flow cytometry analysis showed a significant increase in ecto-5′-NT/CD73 mRNA and protein levels, respectively, comparing confluent with sub-confluent cultures in human U138MG glioma cells. Ecto-5′-nucleotidase/CD73 may regulate the extracellular adenosine 5′-monophosphate (AMP) and adenosine levels. Treatment with 1 μM APCP, a competitive ecto-5′-NT/CD73 inhibitor, caused a significant reduction of 30% in glioma cell proliferation. In addition, 100 μM adenosine increases cell proliferation by 36%, and the treatment with adenosine plus NBTI and dipyridamole, produced an additional and significant increase of on cell proliferation. The inhibitory effect on cell proliferation caused by APCP was reverted by co-treatment with NBTI and dipyridamole. AMP (1 mM and 3 mM) decreased U138MG glioma cell proliferation by 29% and 42%, respectively. Taken together, these results suggest the participation of ecto-5′-NT/CD73 in cell proliferation and that this process is dependent upon the enzyme’s production of adenosine, a proliferative factor, and removal of AMP, a toxic molecule for gliomas.
118 citations
TL;DR: Indomethacin-loaded nanocapsules formulation was more potent than a solution of indometHacin in decreasing the viability and cell proliferation of glioma lines, and selective cytotoxicity of those formulations for tumoral cells was observed.
47 citations
TL;DR: The objective was to detect the presence of nanocrystals in the nanoparticle suspensions using a simple methodology, and to determine if the nanocry crystals are formed during preparation or by drug leakage from the particles during storage.
45 citations
TL;DR: The ability of maternal caffeine intake to interfere on cholinergic neurotransmission during brain development is highlighted by the high score of phosphorylatable residues on acetylcholinesterase, which can be associated with a possible regulation by specific phosphorylation sites.
34 citations
TL;DR: Comparing the extracellular metabolism of AMP and transcriptional levels of the ecto-5'-nucleotidase/CD73 and tissue non-specific alkaline phosphatase in activated and quiescent HSC of the mouse hepatic stellate cell line GRX suggests that ecto -5'- nucleotIDase-dependent adenosine generation may play a role in the regulation of quiescence HSC functions.
24 citations