Andrew C. Briscoe

TL;DR: It is demonstrated that the method of protein extraction, length of handling at room temperature, and repetitive freeze-thaw cycles do not seem to alter the urinary proteome at either the protein or peptide level in a manner that degrades information obtainable by mass spectrometry.

TL;DR: An alternative strategy using a standard domestic microwave oven to perform the de-N-glycosylation of glycoproteins and a complex mixture from human plasma was employed, without any apparent adverse affects on the glycans or protein backbones.

TL;DR: A simple and rapid sample preparation platform, the GlycoFilter, which allows a comprehensive characterization of N- glycans, N-glycosites, and proteins in a single workflow.

TL;DR: A one-step sample preparation workflow that simultaneously concentrates proteins, purifies by removing salts and other low molecular weight compounds, and depletes (albumin) from urine samples is developed that can be multiplexed and compatible with a diverse range of downstream multidimensional separation technologies.

TL;DR: A series of candidate markers for urological chronic pelvic pain syndrome (UCPPS), selected based on their proposed involvement in underlying biological processes so as to provide new insights into pathophysiology and suggest targets for expanded clinical and mechanistic studies.