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Andrew C. Millard
Researcher at University of Connecticut Health Center
Publications - 24
Citations - 2480
Andrew C. Millard is an academic researcher from University of Connecticut Health Center. The author has contributed to research in topics: Second-harmonic imaging microscopy & Second-harmonic generation. The author has an hindex of 15, co-authored 24 publications receiving 2352 citations. Previous affiliations of Andrew C. Millard include University of Connecticut.
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Journal ArticleDOI
Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues.
Paul J. Campagnola,Andrew C. Millard,Mark Terasaki,Pamela E. Hoppe,Christian J. Malone,William A. Mohler +5 more
TL;DR: Second-harmonic imaging microscopy (SHIM) on a laser-scanning system proves, therefore, to be a powerful and unique tool for high-resolution, high-contrast, three-dimensional studies of live cell and tissue architecture.
Journal ArticleDOI
Characterization of the Myosin-Based Source for Second-Harmonic Generation from Muscle Sarcomeres
TL;DR: This study characterize, by biochemical and optical analysis, the critical structures underlying SHG from the complex muscle sarcomere and finds an orientation for the elemental harmonophore that corresponds well to the pitch of the myosin rod alpha-helix along the thick filament axis.
Journal ArticleDOI
Second harmonic generation imaging of endogenous structural proteins.
TL;DR: It is demonstrated that thick tissues including muscle and bone can be imaged and sectioned through several hundred micrometers of depth by laser-scanning second harmonic generation (SHG) microscopy and found that SHG provides molecular level data on radial and lateral symmetries that GFP constructs cannot.
Journal ArticleDOI
Characterization and Application of a New Optical Probe for Membrane Lipid Domains
Lei Jin,Andrew C. Millard,Joseph P. Wuskell,Xuemei Dong,Dianqing Wu,Heather A. Clark,Leslie M. Loew +6 more
TL;DR: The fluorescence of an environmentally sensitive probe for lipid membranes, di-4-ANEPPDHQ, is characterized and a sevenfold stronger second harmonic generation signal in the liquid-disordered domains is observed, consistent with a higher concentration of the dye resulting from preferential partitioning into the disordered phase.
Journal ArticleDOI
Cholesterol-enriched lipid domains can be visualized by di-4-ANEPPDHQ with linear and nonlinear optics.
TL;DR: A membrane-staining dye, di-4-ANEPPDHQ, which differentiates liquid-ordered phases from liquid-disordered phases coexisting in model membranes under both linear and nonlinear microscopies, should lead to broad applications in biophysical studies of lipid domains inmodel membranes and cells.