P
Paul J. Campagnola
Researcher at University of Wisconsin-Madison
Publications - 143
Citations - 9634
Paul J. Campagnola is an academic researcher from University of Wisconsin-Madison. The author has contributed to research in topics: Second-harmonic imaging microscopy & Microscopy. The author has an hindex of 42, co-authored 137 publications receiving 8732 citations. Previous affiliations of Paul J. Campagnola include University of Connecticut & University of Connecticut Health Center.
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Journal ArticleDOI
Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms.
TL;DR: Recent studies of the three-dimensional in vivo structures of well-ordered protein assemblies, such as collagen, microtubules and muscle myosin, are beginning to establish SHIM as a nondestructive imaging modality that holds promise for both basic research and clinical pathology.
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Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues.
Paul J. Campagnola,Andrew C. Millard,Mark Terasaki,Pamela E. Hoppe,Christian J. Malone,William A. Mohler +5 more
TL;DR: Second-harmonic imaging microscopy (SHIM) on a laser-scanning system proves, therefore, to be a powerful and unique tool for high-resolution, high-contrast, three-dimensional studies of live cell and tissue architecture.
Journal ArticleDOI
Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure
Xiyi Chen,Oleg Nadiarynkh,Oleg Nadiarynkh,Sergey V. Plotnikov,Sergey V. Plotnikov,Paul J. Campagnola +5 more
TL;DR: This work discusses how second-harmonic generation microscopy can be used to obtain more structural information on the assembly of collagen in tissues than is possible by other microscopy techniques.
Journal ArticleDOI
High-resolution nonlinear optical imaging of live cells by second harmonic generation.
TL;DR: In this paper, the authors adapted a laser scanning microscope with a titanium sapphire femtosecond pulsed laser and transmission optics to produce live cell images based on the nonlinear optical phenomenon of second harmonic generation (SHG).
Journal Article
High-resolution nonlinear optical imaging of live cells by second harmonic generation
TL;DR: It was found that chirality plays a significant role in the mechanism of contrast generation, and it is also shown that SHIM is highly sensitive to membrane potential, with a depolarization of 25 mV resulting in an approximately twofold loss of signal intensity.