Andrew Timmes

TL;DR: End point dilution RT-QuIC provides a sensitive, rapid, quantitative, and high throughput assay of prion seeding activity and can be accomplished in 2 days or less.

TL;DR: The results demonstrate that the ability to self-propagate into a protease-resistant insoluble conformer is not unique to infectious PrP molecules and suggest that the presence of RNA and lipid cofactors may facilitate the spontaneous refolding of PrP into an infectious form while also allowing the de novo formation ofSelf-propagating, but non-infectious, rPrP-res.

TL;DR: The similarities between PrPSc samples from 22L and Chandler TSE strains suggest that the non‐PrPSc protein components found in standard enrichment protocols are not strain specific.

TL;DR: The results show that contaminating proteins alter the FTIR spectrum attributed to PrPSc and suggest that the α‐helical, loop/turn and β‐sheet secondary structure that remains following their removal are derived from PrP sc itself.

TL;DR: It is shown that another retrovirus, the murine leukemia virus Friend (F-MuLV), also enhanced the release and spread of scrapie infectivity in cell culture, but retroviral co-infection does not necessarily alter prion disease pathogenesis in vivo.