A
Ann Sofie Fält
Researcher at Swedish University of Agricultural Sciences
Publications - 3
Citations - 765
Ann Sofie Fält is an academic researcher from Swedish University of Agricultural Sciences. The author has contributed to research in topics: Gene & Cas9. The author has an hindex of 3, co-authored 3 publications receiving 470 citations.
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Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts.
Mariette Andersson,Helle Turesson,Alessandro Nicolia,Ann Sofie Fält,Mathias Samuelsson,Per Hofvander +5 more
TL;DR: Altered starch quality with full knockout of GBSS gene function in potato was achieved using CRISPR-Cas9 technology, through transient transfection and regeneration from isolated protoplasts, verifying similar results found in other plants that high homology between guide sequence and target region near the protospacer adjacent motif (PAM) site is essential.
Journal ArticleDOI
Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery
Mariette Andersson,Helle Turesson,Niklas Olsson,Ann Sofie Fält,Pia Ohlsson,Matías Nicolás González,Matías Nicolás González,Mathias Samuelsson,Per Hofvander +8 more
TL;DR: This work has implemented a DNA-free genome editing method, using delivery of CRISPR-Cas9 ribonucleoproteins (RNPs) to potato protoplasts, by targeting the gene encoding a granule bound starch synthase (GBSS, EC 2.4.1.242).
Journal ArticleDOI
Reduced Enzymatic Browning in Potato Tubers by Specific Editing of a Polyphenol Oxidase Gene via Ribonucleoprotein Complexes Delivery of the CRISPR/Cas9 System
Matías Nicolás González,Gabriela Alejandra Massa,Gabriela Alejandra Massa,Mariette Andersson,Helle Turesson,Niklas Olsson,Ann Sofie Fält,Leonardo Storani,Cecilia Decima Oneto,Per Hofvander,Sergio Enrique Feingold +10 more
TL;DR: It is demonstrated that the CRISPR/Cas9 system can be applied to develop potato varieties with reduced enzymatic browning in tubers, by the specific editing of a single member of the StPPO gene family.