Antoni Casablancas

TL;DR: The effect of glutamine replacement by glutamate and the balance between glutamate and glucose metabolism on the redistribution of t‐PA‐producing recombinant CHO cells metabolism is studied in a series of glucose shift down and shift up experiments in continuous culture to gain a better insight into cellular metabolism and its redistribution.

TL;DR: The results showed that the combination of CMV promoter and IFNα2 signal peptide resulted highly efficient for recombinant protein production in HEK293 cells.

TL;DR: This work proposes a double limitation strategy (glucose and IPTG) for E. coli fed-batch cultures to avoid the deregulation of the metabolism in the induction phase, and finds that by reducing the concentration of IPTG while keeping glucose growth limitation, the accumulation of acetic acid decreased.

TL;DR: Among the different alternatives studied, the use of THE AUTHORS' on-line monitoring to simultaneously determine cell growth and metabolic activity appears to be the best method to adjust substrate feeding owing to its simplicity and robustness.

TL;DR: Comparisons of two different monitoring tools for indirect estimation of biomass concentration in a HEK293 cell cultures producing IFN-γ prove that fed-batch strategy based on the alkali buffer addition is a robust online monitoring method that has shown its great potential to optimize the feeding strategy inHEK293 fed- batches.