Several algorithms have been described in the literature for protein identification by searching a sequence database using mass spectrometry data. In some approaches, the experimental data are peptide molecular weights from the digestion of a protein by an enzyme. Other approaches use tandem mass spectrometry (MS/MS) data from one or more peptides. Still others combine mass data with amino acid sequence data. We present results from a new computer program, Mascot, which integrates all three types of search. The scoring algorithm is probability based, which has a number of advantages: (i) A simple rule can be used to judge whether a result is significant or not. This is particularly useful in guarding against false positives. (ii) Scores can be compared with those from other types of search, such as sequence homology. (iii) Search parameters can be readily optimised by iteration. The strengths and limitations of probability-based scoring are discussed, particularly in the context of high throughput, fully automated protein identification.

Probability-based protein identification by searching sequence databases using mass spectrometry data.

http://nhjy.hzau.edu.cn/kech/ssyy/pdf/drought/4.PDF

Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell

The three-dimensional structure of acetylcholinesterase from Torpedo californica electric organ has been determined by x-ray analysis to 2.8 angstrom resolution. The form crystallized is the glycolipid-anchored homodimer that was purified subsequent to solubilization with a bacterial phosphatidylinositol-specific phospholipase C. The enzyme monomer is an alpha/beta protein that contains 537 amino acids. It consists of a 12-stranded mixed beta sheet surrounded by 14 alpha helices and bears a striking resemblance to several hydrolase structures including dienelactone hydrolase, serine carboxypeptidase-II, three neutral lipases, and haloalkane dehalogenase. The active site is unusual because it contains Glu, not Asp, in the Ser-His-acid catalytic triad and because the relation of the triad to the rest of the protein approximates a mirror image of that seen in the serine proteases. Furthermore, the active site lies near the bottom of a deep and narrow gorge that reaches halfway into the protein. Modeling of acetylcholine binding to the enzyme suggests that the quaternary ammonium ion is bound not to a negatively charged "anionic" site, but rather to some of the 14 aromatic residues that line the gorge.

https://science.sciencemag.org/content/sci/253/5022/872.full.pdf

Atomic structure of acetylcholinesterase from Torpedo californica: a prototypic acetylcholine-binding protein

Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters

ROS signaling: the new wave?

Immunological detection of NADH-specific enoyl-ACP reductase from rape seed (Brassica napus) — induction, relationship of α and β polypeptides, mRNA translation and interaction with ACP

In an attempt to isolate a plant malonyl-coenzyme A:acyl carrier protein transacylase cDNA clone, by direct genetic selection in an Escherichia coli fabD mutant (LA2-89) with a maize cDNA expression library, a Zea mays cDNA clone encoding a GTP-binding protein of the ARF family was isolated. Complementation of a mutation affecting bacterial membrane lipid biosynthesis by a plant ARF protein, could indicate the existence of as yet unidentified bacterial equivalents of this ubiquitous eucaryotic GTP-binding protein.

A Zea mays GTP-binding protein of the ARF family complements an Escherichia coli mutant with a temperature-sensitive malonyl-coenzyme A:acyl carrier protein transacylase.

Les lipides jouent un role essentiel dans la biologie cellulaire. Cet article a pour but de rapporter les connaissances actuelles sur la biosynthese des lipides dans les plantes et de definir quelle recherche il serait important d'entreprendre dans l'avenir. Le recent sequencage du genome entier de l'Arabidopsis (plante de la famille de cruciferes comme le colza) constitue un outil puissant pour les chercheurs qui etudient la biosynthese de l'huile et sa regulation.

Biosynthesis and regulation of fatty acids and triglycerides in oil seed rape. Current status and future trends

A Novel Function for Arabidopsis CYCLASE1 in Programmed Cell Death Revealed by Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) Analysis of Extracellular Matrix Proteins

Disclosed is a nucleotide sequence encoding an enzyme having acyl-ACP thioesterase activity comprising nucleotides 169-1269 of the sequence shown in Figure 1 or functional equivalents thereof. Also disclosed is a polypeptide possessing said enzyme activity, vectors, host cells, and transgenic plants comprising the novel nucleotide sequence, together with a method of altering the characteristics of a plant and a method of producing the enzyme.

Antoni R. Slabas

Papers

Immunological detection of NADH-specific enoyl-ACP reductase from rape seed (Brassica napus) — induction, relationship of α and β polypeptides, mRNA translation and interaction with ACP

A Zea mays GTP-binding protein of the ARF family complements an Escherichia coli mutant with a temperature-sensitive malonyl-coenzyme A:acyl carrier protein transacylase.

Biosynthesis and regulation of fatty acids and triglycerides in oil seed rape. Current status and future trends

A Novel Function for Arabidopsis CYCLASE1 in Programmed Cell Death Revealed by Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) Analysis of Extracellular Matrix Proteins

Recombinant plant enzyme