Antonio Paneque

TL;DR: The in vivo and in vitro reactivation of nitrate reductase and the derepression of all the enzymes of the nitrate reducing system are also described.

TL;DR: Nitrite reductase from a crude homogenate of spinach leaves has been purified about 500-fold and freed from NADP-reductase, and a method based on the reduction of nitrite by ferredoxin chemically reduced with hydrosulfite has been successfully applied to the aerobic assay of the enzyme.

TL;DR: Under conditions which exclude de novo synthesis of nitrate reductase, the addition of molybdate to moly bdenum-deficient cells clearly increases the activity level of this enzyme, thus suggesting in vivo incorporation of the trace metal into the pre-existing inactive apoenzyme.

TL;DR: Due to the fact that FMN and FAD are the effective electron donors for the reduction of NO3− in higher plants and that they can be reduced by a variety of enzyme systems, the enzyme until now known as NAD(P)H2: nitrate oxidoreductase has to be considered as a mixture of two different proteins, and the latter will be systematically classified as FMNH2(FADH2): nitrate reductase.

TL;DR: In the green alga Chlamydomonas reinhardi, all the enzymes of the nitrate-reducing system are nutritionally repressed by ammonia as discussed by the authors.