A
Arndt Brachat
Researcher at University of Basel
Publications - 9
Citations - 8992
Arndt Brachat is an academic researcher from University of Basel. The author has contributed to research in topics: Gene & Green fluorescent protein. The author has an hindex of 9, co-authored 9 publications receiving 8567 citations.
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Journal ArticleDOI
Time-lapse video microscopy analysis reveals astral microtubule detachment in the yeast spindle pole mutant cnm67.
TL;DR: It is postulate that in cnm67Delta cells Spc72-gamma-tubulin complex-capped astral microtubules are released from the half-bridge upon SPB separation but fail to be anchored to the cytoplasmic side of the SPB because of the absence of an outer plaque.
Book ChapterDOI
5 PCR-Based Gene Targeting in Saccharomyces cerevisiae
Achim Wach,Arndt Brachat,Corinne Rebischung,Sabine Steiner,Karine Pokorni,Stephan te Heesen,Peter Philippsen +6 more
TL;DR: This chapter discusses the different steps of the polymerase chain reaction (PCR)-based gene targeting method and its possible applications for basic and advanced gene analyses and highlights the experience with the selection marker, kanMX, a hybrid gene expressing a bacterial aminoglycoside phosphotransferase under the control of a strong fungal promoter.
Journal ArticleDOI
Reorientation of Mispositioned Spindles in Short Astral Microtubule Mutant spc72Δ Is Dependent on Spindle Pole Body Outer Plaque and Kar3 Motor Protein
TL;DR: It is shown that cells lacking Spc72, the SPB receptor of the cytoplasmic gamma-tubulin complex, can only generate very short (<1 microm) and unstable astral microtubules, and that spindle reorientation was most likely accomplished by cortex interactions of the very short astralmicrotubules.
Journal ArticleDOI
Analysis of deletion phenotypes and GFP fusions of 21 novel Saccharomyces cerevisiae open reading frames.
Arndt Brachat,Nicole Liebundguth,Corinne Rebischung,Sophie Lemire,Sophie Lemire,Florian SchÄrer,Dominic Hoepfner,Vasyl Demchyshyn,Vasyl Demchyshyn,Isabelle Howald,Andreas Düsterhöft,Dörte Möstl,Rainer Pöhlmann,Peter Kötter,Michael N. Hall,Achim Wach,Peter Philippsen +16 more
TL;DR: Green fluorescent protein (GFP) tagging was performed on 21 novel yeast open reading frames investigated by growth and sporulation tests of deletion mutants and obtained a discernible GFP localization pattern in five of 20 strains investigated.