Showing papers by "Arno G. Motulsky published in 1966"
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62 citations
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TL;DR: This finding suggests that the production of ε-chains initially exceeds that of other known hemoglobin chains, and that hemoglobin Gower 1 is the predominant hemoglobin in early human embryonic life.
Abstract: Hemoglobin Gower 1, the structure of which is thought to be epsilon(4), is the predominant hemoglobin in early human embryonic life. This finding suggests that the production of (epsilon)-chains initially exceeds that of other known (alpha, beta, gamma, and delta) chains.
51 citations
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34 citations
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TL;DR: A hitherto unknown inherited hemoglobin variant, Hb Tacoma, was discovered in three healthy members of a family of European extraction and is associated with a raised Hb A2 level without evidence of β-thalassemia.
Abstract: A hitherto unknown inherited hemoglobin variant, Hb Tacoma, was discovered in three healthy members of a family of European extraction. Hybridization experiments with canine hemoglobin indicated a structural abnormality in the β-chain. The variant was therefore designated as Hb α2 β2
Tacoma. Separation of Hb Tacoma from Hb A could only be clearly achieved by starch grain electrophoresis in TEB buffer at pH 8.6–9.0, where Hb Tacoma moved more rapidly towards the anode than Hb A; gradient column chromatography on DEAE cellulose and CM-Sephadex achieved partial separation. The proportion of abnormal hemoglobin in the heterozygote amounted to 43 per cent of the total hemoglobin. Hb Tacoma was less heat resistant and became more rapidly denatured in 8 M urea solution than Hb A, Hb C, or Hb S. On thin-layer starch gel electrophoresis in TCB buffer at pH 8.6 Hb Tacoma was associated with an electrophoretically slow, benzidine-positive component, Hb Tacoma-slow. The exact biochemical nature of this minor component could not be determined, although a polymeric product of Hb Tacoma is suspected. Heterozygosity for Hb Tacoma is associated with a raised Hb A2 level without evidence of β-thalassemia.
31 citations
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TL;DR: The clinical significance of the deficiency of red-cell glucose-6-phosphate dehydrogenase was realized when this enzymatic abnormality was found to be the basic lesion responsible for the deficiency.
Abstract: Excerpt The clinical significance of the deficiency of red-cell glucose-6-phosphate dehydrogenase (G-6-PD) was realized when this enzymatic abnormality was found to be the basic lesion responsible ...
29 citations
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TL;DR: These findings account for the absence of elevated hemoglobin A2 in this form of thalassemia and demonstrate that (δ β) thalasmia is an entity genetically distinct from A2 thalasamia.
21 citations
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3 citations
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TL;DR: Since hereditary spherocytosis in deer mice in a temperate climate is compatible with good health, these findings provide a further illustration of interaction of a particular environmental insult with a specific genotype in the pathogenesis of hereditary disease.
3 citations