Showing papers by "Arun K. Bhunia published in 2017"
TL;DR: Oil-in-water emulsions formed using starch octenyl succinate were used to stabilize nisin and thymol in cantaloupe juice-containing fluid and displayed much greater effect to inhibit Listeria and Salmonella than non-emulsion, aqueous formulations.
41 citations
TL;DR: The results showed that theBiofilms of both bacteria substantially reduced LAE efficacy, and that the biofilm of L. monocytogenes was more resistant than that of S. Typhimurium.
24 citations
TL;DR: Though low hydrophobicity and high number of charges help in the formation of water channel for transmembrane aggregates, insertion of peptides with these properties requires overcome of energy barrier, thereby resulting in low antimicrobial activity.
18 citations
TL;DR: Cysteine-terminus modified Cecropin P1 (CP1C, sequence SWLSTAKKLENSAKKRLSEGIAIAIQGGPRC) was chemically immobilized onto silica nanoparticles with maleimide-PEG-NHS ester cross-linkers of different PEG chain lengths to explain the loss of antimicrobial activity of tethered CP1C.
18 citations
TL;DR: A multispectral elastic-light-scatter instrument that can simultaneously detect three-wavelength scatter patterns and associated optical densities from individual bacterial colonies is reported, overcoming the limits of the single-wa wavelength predecessor.
Abstract: Three wavelength-merged scatter pattern from E. coli.
We report a multispectral elastic-light-scatter instrument that can simultaneously detect three-wavelength scatter patterns and associated optical densities from individual bacterial colonies, overcoming the limits of the single-wavelength predecessor. Absorption measurements on liquid bacterial samples revealed that the spectroscopic information can indeed contribute to sample differentiability. New optical components, including a pellicle beam splitter and an optical cage system, were utilized for robust acquisition of multispectral images. Four different genera and seven shiga toxin producing E. coli serovars were analyzed; the acquired images showed differences in scattering characteristics among the tested organisms. In addition, colony-based spectral optical-density information was also collected. The optical model, which was developed using diffraction theory, correctly predicted wavelength-related differences in scatter patterns, and was matched with the experimental results. Scatter-pattern classification was performed using pseudo-Zernike (GPZ) polynomials/moments by combining the features collected at all three wavelengths and selecting the best features via a random-forest method. The data demonstrate that the selected features provide better classification rates than the same number of features from any single wavelength.
17 citations
TL;DR: The study showed the low efficacy of LAE and SHC on cantaloupe rind surface to reduce the E. coli biofilm, suggesting the needs for cantalOUpe cleaning methods beyond washing with conventional antimicrobial agents.
16 citations
TL;DR: In this article, the authors performed whole-genome sequencing of L. monocytogenes strain F4244 (serotype 4b) using Illumina sequencing, and the sequence showed 94.5% identity with strain F2365, serotype 4 b, and 90.6% with EGD-e and serotype 1/2a.
Abstract: Listeria monocytogenes is an opportunistic invasive foodborne pathogen. Here, we performed whole-genome sequencing of L. monocytogenes strain F4244 (serotype 4b) using Illumina sequencing. The sequence showed 94.5% identity with strain F2365, serotype 4b, and 90.6% with EGD-e, serotype 1/2a.
10 citations
01 Jan 2017
TL;DR: This chapter will highlight both wild-type and bioengineered probiotic strains in order to control foodborne illness, and proposed modes of action will be expanded upon.
Abstract: Foodborne illness is a serious global health concern. There are over 200 known microbial, chemical, and physical agents that have the potential to cause foodborne illness. There have been several advances in foodborne pathogen control and prevention, which include antibiotics, antimicrobial molecules, ionizing particles, ultraviolet radiation, and heat; however, foodborne illness remains a critical problem to the world’s food supply. Probiotics have been used for over 200 years to promote, not only general, but also gastrointestinal health. These probiotics offer a unique strategy for control and prevention of foodborne illness, while conferring the same additional health benefits that probiotics have been known to confer for years. This chapter will highlight both wild-type and bioengineered probiotic strains in order to control foodborne illness. Further, proposed modes of action will also be expanded upon. While probiotics hold promise as strategies for foodborne pathogen control and treatment, challenges remain in the realm of characterization, administration and dosing, as well as disparities in host-strain specificities.
3 citations
TL;DR: The authors wish to correct the oligonucleotide sequence of primer E-LAP-F1 and LIS-R1 in Table 1 in their paper published in Sensors.
Abstract: The authors wish to correct the oligonucleotide sequence of primer E-LAP-F1 and LIS-R1 in Table 1in their paper published in Sensors [1], doi:10.3390/s150922672, http://www.mdpi.com/1424-8220/15/9/22672. The following table should be used.[...].
1 citations