A
Asher Williams
Researcher at Rensselaer Polytechnic Institute
Publications - 16
Citations - 275
Asher Williams is an academic researcher from Rensselaer Polytechnic Institute. The author has contributed to research in topics: Sulfation & Chondroitin. The author has an hindex of 7, co-authored 14 publications receiving 173 citations. Previous affiliations of Asher Williams include New York University.
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Journal ArticleDOI
The road to animal-free glycosaminoglycan production: current efforts and bottlenecks.
TL;DR: It is envisioned that new engineering approaches together with advances in the basic biology and chemistry of GAGs will move GAG production beyond its currently limited supply chain.
Journal ArticleDOI
Engineered heparins as new anticoagulant drugs.
Deepika Vaidyanathan,Asher Williams,Jonathan S. Dordick,Mattheos A. G. Koffas,Robert J. Linhardt +4 more
TL;DR: This review examines progress toward the preparation of engineered heparins through chemical synthesis, chemoenzymatic synthesis, and metabolic engineering.
Journal ArticleDOI
Heavy Heparin: A Stable Isotope-Enriched, Chemoenzymatically-Synthesized, Poly-Component Drug.
Brady F. Cress,Ujjwal Bhaskar,Deepika Vaidyanathan,Asher Williams,Chao Cai,Xinyue Liu,Li Fu,Vandhana M‐Chari,Fuming Zhang,Shaker A. Mousa,Jonathan S. Dordick,Mattheos A. G. Koffas,Robert J. Linhardt +12 more
TL;DR: This work chemoenzymatically synthesized perdeuteroheparin from biosynthetically enriched heparosan precursor obtained from microbial culture in deuterated medium, and expects that this approach can be extended to produce other isotope-enriched glycosaminoglycans.
Journal ArticleDOI
Complete biosynthesis of a sulfated chondroitin in Escherichia coli.
Abinaya Badri,Asher Williams,Adeola Awofiranye,Payel Datta,Ke Xia,Wenqin He,Keith Fraser,Jonathan S. Dordick,Robert J. Linhardt,Mattheos A. G. Koffas +9 more
TL;DR: In this paper, the authors demonstrate the development of single microbial cell factories capable of complete, one-step biosynthesis of chondroitin sulfate (CS), a type of GAG.
Journal ArticleDOI
Increased 3'-Phosphoadenosine-5'-phosphosulfate Levels in Engineered Escherichia coli Cell Lysate Facilitate the In Vitro Synthesis of Chondroitin Sulfate A.
TL;DR: A 10.4-fold increase in the amount of CS produced by biotransformation is shown by employing PAPS from the engineered PAPS-accumulating strain, and for the first time, in vitro biOTransformation of CS using PAPS, chondroitin and chondDetroitin-4-sulfotransferase (C4ST) all synthesized from different engineered E. coli strains made in the laboratory.