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Aya Shibamiya
Researcher at University of Hamburg
Publications - 7
Citations - 650
Aya Shibamiya is an academic researcher from University of Hamburg. The author has contributed to research in topics: Regenerative medicine & Genetic enhancement. The author has an hindex of 5, co-authored 7 publications receiving 502 citations.
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Journal ArticleDOI
Human engineered heart tissue as a versatile tool in basic research and preclinical toxicology.
Sebastian Schaaf,Aya Shibamiya,Marco Mewe,Alexandra Eder,Andrea Stöhr,Marc N. Hirt,Thomas Rau,Wolfram-Hubertus Zimmermann,Lenard Conradi,Thomas Eschenhagen,Arne Hansen +10 more
TL;DR: This study establishes hEHT as a simple in vitro model for heart research and describes a simple and robust protocol for the generation of fibrin-based human engineered heart tissue in a 24-well format using an unselected population of differentiated human embryonic stem cells containing 30–40% α-actinin-positive cardiac myocytes.
Journal ArticleDOI
Differentiation of cardiomyocytes and generation of human engineered heart tissue
K. Breckwoldt,David Letuffe-Brenière,Ingra Mannhardt,Thomas G. Schulze,Bärbel M. Ulmer,Tessa Werner,Anika Benzin,Birgit Klampe,Marina Reinsch,Sandra D. Laufer,Aya Shibamiya,Maksymilian Prondzynski,Giulia Mearini,Dennis Schade,Sigrid Fuchs,Christiane Neuber,Elisabeth Krämer,Umber Saleem,Mirja L Schulze,Marita L. Rodriguez,Thomas Eschenhagen,Arne Hansen +21 more
TL;DR: This protocol describes the differentiation of cardiomyocytes from hiPSCs; standardized generation of defined embryoid bodies, growth factor and small-molecule-based cardiac differentiation; and standardizedgeneration of EHTs.
Journal ArticleDOI
Evaluation of MYBPC3 trans-Splicing and Gene Replacement as Therapeutic Options in Human iPSC-Derived Cardiomyocytes.
Maksymilian Prondzynski,Elisabeth Krämer,Sandra D. Laufer,Aya Shibamiya,Ole Pless,Frederik Flenner,Oliver J. Müller,Oliver J. Müller,Julia Münch,Charles Redwood,Arne Hansen,Monica Patten,Thomas Eschenhagen,Giulia Mearini,Lucie Carrier +14 more
TL;DR: Evidence is provided for the feasibility of trans-splicing, although with low efficiency, and efficient gene replacement in hiPSC-CMs with a MYBPC3 mutation, which restored the cMyBP-C level to 81% of the control level, suppressed hypertrophy, and partially restored gene expression to control level in HCM cells.
Journal ArticleDOI
Human Engineered Heart Tissue Patches Remuscularize the Injured Heart in a Dose-Dependent Manner
Eva Querdel,Marina Reinsch,L. Castro,Deniz Köse,Andrea Bähr,Andrea Bähr,Svenja Reich,Birgit Geertz,Bärbel M. Ulmer,Mirja L Schulze,Marc D Lemoine,Tobias Krause,Marta Lemme,Jascha Sani,Aya Shibamiya,Tim Stüdemann,Maria Köhne,Constantin V. von Bibra,Nadja Hornaschewitz,Simon Pecha,Yusuf Nejahsie,Ingra Mannhardt,Torsten Christ,Hermann Reichenspurner,Arne Hansen,Nikolai Klymiuk,Nikolai Klymiuk,Markus Krane,Christian Kupatt,Thomas Eschenhagen,Florian Weinberger +30 more
TL;DR: In this article, human engineered heart tissue (EHT) transplantation represents a potential regenerative strategy for patients with heart failure and has been successful in preclinical models. Clinical...
Journal ArticleDOI
Cell Banking of hiPSCs: A Practical Guide to Cryopreservation and Quality Control in Basic Research
Aya Shibamiya,Elisabeth Schulze,Dana Krauß,Dana Krauß,Christa Augustin,Marina Reinsch,Mirja L Schulze,Simone Steuck,Giulia Mearini,Ingra Mannhardt,Thomas G. Schulze,Birgit Klampe,Tessa Werner,Umber Saleem,Anika E Knaust,Sandra D. Laufer,Christiane Neuber,Marta Lemme,Charlotta Sophie Behrens,Malte Loos,Florian Weinberger,Sigrid Fuchs,Thomas Eschenhagen,Arne Hansen,Bärbel M. Ulmer +24 more
TL;DR: A cost-efficient route to generate master and working-cell banks for basic research projects and minimal protocols for quality assurance including tests for sterility, viability, pluripotency, and genetic integrity are described.