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Christiane Neuber
Researcher at University of Hamburg
Publications - 16
Citations - 1241
Christiane Neuber is an academic researcher from University of Hamburg. The author has contributed to research in topics: Protein phosphatase 1 & Protein kinase A. The author has an hindex of 11, co-authored 16 publications receiving 994 citations.
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Journal ArticleDOI
Functional improvement and maturation of rat and human engineered heart tissue by chronic electrical stimulation.
Marc N. Hirt,Jasper Boeddinghaus,Alice Mitchell,Sebastian Schaaf,Christian Börnchen,Christian Müller,Herbert Schulz,Herbert Schulz,Norbert Hubner,Norbert Hubner,Justus Stenzig,Andrea Stoehr,Christiane Neuber,Alexandra Eder,Pradeep K. Luther,Arne Hansen,Thomas Eschenhagen +16 more
TL;DR: A novel pacing system integrated in a setup for videooptical recording of EHT contractile function over time and whether sustained electrical field stimulation improved EHT properties appeared to be an important step toward the generation of fully mature EHT.
Journal ArticleDOI
Human Engineered Heart Tissue: Analysis of Contractile Force
Ingra Mannhardt,K. Breckwoldt,David Letuffe-Brenière,Sebastian Schaaf,Herbert Schulz,Christiane Neuber,Anika Benzin,Tessa Werner,Alexandra Eder,Thomas G. Schulze,Birgit Klampe,Torsten Christ,Marc N. Hirt,Norbert Huebner,Alessandra Moretti,Thomas Eschenhagen,Arne Hansen +16 more
TL;DR: Systematic contractility analysis reveals that EHTs replicated canonical response to physiological and pharmacological regulators of inotropy, membrane- and calcium-clock mediators of pacemaking, modulators of ion-channel currents, and proarrhythmic compounds with unprecedented precision.
Journal ArticleDOI
Differentiation of cardiomyocytes and generation of human engineered heart tissue
K. Breckwoldt,David Letuffe-Brenière,Ingra Mannhardt,Thomas G. Schulze,Bärbel M. Ulmer,Tessa Werner,Anika Benzin,Birgit Klampe,Marina Reinsch,Sandra D. Laufer,Aya Shibamiya,Maksymilian Prondzynski,Giulia Mearini,Dennis Schade,Sigrid Fuchs,Christiane Neuber,Elisabeth Krämer,Umber Saleem,Mirja L Schulze,Marita L. Rodriguez,Thomas Eschenhagen,Arne Hansen +21 more
TL;DR: This protocol describes the differentiation of cardiomyocytes from hiPSCs; standardized generation of defined embryoid bodies, growth factor and small-molecule-based cardiac differentiation; and standardizedgeneration of EHTs.
Journal ArticleDOI
Human iPSC-derived cardiomyocytes cultured in 3D engineered heart tissue show physiological upstroke velocity and sodium current density
Marc D Lemoine,Ingra Mannhardt,K. Breckwoldt,Maksymilian Prondzynski,Frederik Flenner,Bärbel M. Ulmer,Marc N. Hirt,Christiane Neuber,András Horváth,András Horváth,Benjamin Kloth,Hermann Reichenspurner,Stephan Willems,Arne Hansen,Thomas Eschenhagen,Torsten Christ +15 more
TL;DR: The EHT format promotes INa density and action potential upstroke velocity of hiPSC-CM towards adult values, indicating its usefulness as a model for excitability of human cardiac tissue.
Journal ArticleDOI
Automated analysis of contractile force and Ca2+ transients in engineered heart tissue.
Andrea Stoehr,Christiane Neuber,Christina Baldauf,Ingra Vollert,Felix W. Friedrich,Frederik Flenner,Lucie Carrier,Alexandra Eder,Sebastian Schaaf,Marc N. Hirt,Bülent Aksehirlioglu,Carl W. Tong,Alessandra Moretti,Thomas Eschenhagen,Arne Hansen +14 more
TL;DR: An automated technology to perform sequential analysis of contractile force and Ca(2+) transients in up to 11 strip-format, fibrin-based rat, mouse, and human fura-2-loaded engineered heart tissues (EHTs) under perfusion and electrical stimulation is described.