Barid B. Mukherjee

TL;DR: The ferricyanide reduction assay described provides an estimate of the activity of the α-ketoglutarate dehydrogenase component (E1) of the complex, the only reliable assay for the intact complex however it is not suitable for crude preparations that contain DPNH oxidase.

TL;DR: The concept of a multifunctional role for OPN in bone formation and remodelling is supported, with the peak expression of OPN later in bone development, together with high expression at sites of rapid remodelling indicate that OPN deposited on the surface of mineralized connective tissues may provide a template for osteoclastic resorption.

TL;DR: It is found that cultured CASMCs during log phase of growth and smooth muscle cell layer of the coronary atherosclerotic tissues of patients express both OPN mRNA and protein at a significantly elevated level compared with controls, which raises the possibility that transient inhibition of OPN gene expression or blocking of Opn-αvβ3 interaction may provide a therapeutic approach to preventing restenosis.

TL;DR: Evidence is provided for possible functional roles of 69-kDa osteopontin and suggest that its physiological properties are regulated by post-translational modifications.

TL;DR: It is reported here that 2ar is also related to pp69, a major phosphoprotein secreted by normal rat kidney cells, and enhanced secretion of 2ar/pp69/osteopontin by transformation of a wide variety of mammalian fibroblasts and epithelial cells is often correlated with tumorigenicity.