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Benjamin Flottmann
Researcher at Heidelberg University
Publications - 9
Citations - 325
Benjamin Flottmann is an academic researcher from Heidelberg University. The author has contributed to research in topics: Super-resolution microscopy & Microscopy. The author has an hindex of 9, co-authored 9 publications receiving 299 citations. Previous affiliations of Benjamin Flottmann include Goethe University Frankfurt.
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Journal ArticleDOI
Chemically Induced Photoswitching of Fluorescent Probes—A General Concept for Super-Resolution Microscopy
Ulrike Endesfelder,Sebastian Malkusch,Benjamin Flottmann,Justine Mondry,Piotr Liguzinski,Peter J. Verveer,Mike Heilemann +6 more
TL;DR: Fluorescence probes that can be photoswitched or photoactivated and are suited for single-molecule localization based super-resolution microscopy are reviewed, and mEos2 is identified as a fluorescent protein that exhibits reversible photoswitching under various imaging buffer conditions.
Journal ArticleDOI
Three-Dimensional, Tomographic Super-Resolution Fluorescence Imaging of Serially Sectioned Thick Samples
TL;DR: TomoSTORM, an approach combining single-molecule localization-based super-resolution microscopy with array tomography of structurally intact brain tissue, delineated the course of the membrane and fine-structure of mitochondria in large tissue volumes with a resolution three orders of magnitude better than confocal microscopy.
Journal ArticleDOI
Single‐molecule super‐resolution imaging by tryptophan‐quenching‐induced photoswitching of phalloidin‐fluorophore conjugates
Siddharth Nanguneri,Benjamin Flottmann,Frank Herrmannsdörfer,Kuner Thomas,Mike Heilemann,Mike Heilemann +5 more
TL;DR: The nanoenvironment of fluorophores within a probe can be advantageously used to induce particular properties such as light‐induced photoswitching and single‐molecule super‐resolution imaging for various fluorophore‐phalloidin conjugates in aqueous buffer without the addition of further chemicals.
Journal ArticleDOI
Kar1 binding to Sfi1 C-terminal regions anchors the SPB bridge to the nuclear envelope
Christian Seybold,Menattallah Elserafy,Diana Rüthnick,Musa Ozboyaci,Annett Neuner,Benjamin Flottmann,Mike Heilemann,Mike Heilemann,Rebecca C. Wade,Elmar Schiebel +9 more
TL;DR: Kar1 interacts with the C-terminal (CT) region of Sfi1 to tether the yeast centrosome’s bridge to the nuclear envelope; Sfi 1-CT and C- terminal Cdc31-binding regions form the antiparallel Sfi2 overlap in the bridge.
Journal ArticleDOI
Correlative light microscopy for high-content screening.
Benjamin Flottmann,Manuel Gunkel,Tautvydas Lisauskas,Mike Heilemann,Vytaute Starkuviene,Jürgen Reymann,Holger Erfle +6 more
TL;DR: This work introduces correlative light microscopy by combining fast automated widefield imaging, confocal microscopy and super-resolution microscopy, and demonstrates the potential of this approach for scalable experiments.