Bikas Vaidya

TL;DR: Low-density arrays were assembled into microfluidic channels hot-embossed in poly(methyl methacrylate) (PMMA) to allow the detection of low-abundant mutations in gene fragments that carry point mutations with high diagnostic value for colorectal cancers to reduce the hybridization time from 3 h for a conventional array to less than 1 min.

TL;DR: In this paper, chemical modification of poly(methyl methacrylate) (PMMA), and poly(carbonate) (PC) surfaces for applications in microfluidic systems was described.

TL;DR: The ability to form patterns of chemically reactive surface functionalities in microanalytical devices using a simple photopatterning approach without the need for photoresist-based methods is described.

TL;DR: The photochemical surface modification of poly(methyl methacrylate), PMMA, microfluidic devices by UV light to yield pendant carboxylic acid surface moieties is reported, finding that the water contact angle on PMMA surfaces decreases after exposure to UV light, but upon rinsing with 2-propanol, the water contacts increase.

TL;DR: The PC-SPRI method was shown to effectively remove excess dye terminator from the CGE tract, but yielded lower plate numbers, as compared to a direct injection method with purification accomplished off-chip, with loss in efficiency found to result primarily from the extended injection time associated with the microchip purification method.