Björn Frahm

TL;DR: In this paper, the effect of both conventional and genetic engineering techniques on cell yield and antibody production of two NS0 cell lines is presented, and the adaptive model-based OLFO controller, developed as a general tool for cell culture fed-batch processes, was able to control the fedbatch and dialysis fedbatch cultivations of both cell lines.

TL;DR: A mathematical method to utilize off-gas measurement and culture pH for cell suspension culture is presented, which takes pH changes, buffer and medium characteristics that effect CO(2) mass transfer into account and allows the determination of the respiratory activity of the cells.

TL;DR: The combination of a fed‐batch process and an Open‐Loop‐Feedback‐Optimal (OLFO) control provides a new approach for cell culture process control that couples an efficient cultivation concept to a capable process control strategy.

TL;DR: The dynamic membrane aeration reactor is well suited for the bubbleless oxygenation of laccase-regenerated dehydrogenase reactions and other oxidase-catalyzed reactions on large scale to perform sustainable enzymatic oxidation reactions employing enzymes sensitive to shear or the gas/liquid interface.

TL;DR: The present paper illustrates the advantages and disadvantages of different fed‐batch strategies, including fixed‐feed trajectories, control via THE AUTHORS' (oxygen uptake rate), a priori determination of feed trajectories based on a kinetic model and the model‐based adaptive OLFO (open‐loop‐feedback‐optimal) control strategy.