Brian Minie

TL;DR: An automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced is presented.

TL;DR: Chang et al. as mentioned in this paper developed a high-throughput Chromatin ImmunoPrecipitation (HT-ChIP) method to systematically map protein-DNA interactions to define the dynamics of DNA binding by 25 transcription factors and 4 chromatin marks at 4 time-points following pathogen stimulus of dendritic cells.

TL;DR: This unit describes a protocol for the targeted enrichment of exons from randomly sheared genomic DNA libraries using an in‐solution hybrid selection approach for sequencing on an Illumina Genome Analyzer II.

TL;DR: A high-throughput Chromatin ImmunoPrecipitation (HT-ChIP) method is developed to systematically map protein-DNA interactions and suggests a model for transcription regulation whereby TF networks are hierarchically organized into cell differentiation factors, factors that bind targets prior to stimulus to prime them for induction, and factors that regulate specific gene programs.