Ronnie Blecher-Gonen

TL;DR: A combination of tissue- and lineage-specific transcription factors form the regulatory networks controlling chromatin specification in tissue-resident macrophages, and the environment is capable of shaping the chromatin landscape of transplanted bone marrow precursors, and even differentiated macrophage can be reprogrammed when transferred into a new microenvironment.

TL;DR: The findings uncover a dichotomous molecular function for the reprogramming factors, serving to reactivate endogenous pluripotency networks while simultaneously directly recruiting the Mbd3/NuRD repressor complex that potently restrains the reactivation of OSKM downstream target genes.

TL;DR: Chang et al. as mentioned in this paper developed a high-throughput Chromatin ImmunoPrecipitation (HT-ChIP) method to systematically map protein-DNA interactions to define the dynamics of DNA binding by 25 transcription factors and 4 chromatin marks at 4 time-points following pathogen stimulus of dendritic cells.

TL;DR: Viral-Track is introduced, a computational method that globally scans unmapped scRNA-seq data for the presence of viral RNA, enabling transcriptional cell sorting of infected versus bystander cells and provides a robust technology for dissecting the mechanisms of viral-infection and pathology.

TL;DR: By using paramagnetic beads, this protocol streamline the entire ChIP and indexed library construction process: sample transfer and loss is minimized and the need for manually labor-intensive procedures such as washes, gel extraction and DNA precipitation is eliminated.