C. Hirth

TL;DR: A random primed expression cDNA library was constructed from the RNA of NG 108-15 cells that encodes a 371-amino acid-residue protein presenting all the structural characteristics of receptors that interact with guanine nucleotide-binding proteins.

TL;DR: The structural and chemical data show the important role of aromatic groups as binding sites for quaternary ligands in Torpedo acetylcholinesterase, and provide complementary evidence assigning Trp-84 and Phe-330 to the "anionic" subsite of the active site andtrp-279 to the 'peripheral' anionic site.

TL;DR: The acetylcholine‐binding site on the native AChR may involve several distinct portions of the α‐chain, with the region α 179–207 making a major contribution to the site.

TL;DR: Specific photoaffinity labelling of purified electric eel acetylcholinesterase by 3H‐labelled p‐(N,N‐dimethyl‐amino) benzenediazonium fluoroborate allows the identification of a labelled peptide fragment which is described as being involved in the binding of quaternary ammonium ions on this enzyme.

TL;DR: The molecular environment of a snake neurotoxin interacting with the high- and low-affinity binding sites of the nicotinic acetylcholine receptor (AcChoR) is defined by photocoupling reactions using three toxin derivatives with photoactivatable moieties on Lys-15, Lys-47, and Lys-51.