Carla P. Guimaraes

TL;DR: In this article, the authors exploit bacterial sortases to attach a variety of moieties to the capsid proteins of M13 bacteriophage, which can be functionalized with entities ranging from small molecules (e.g., fluorophores, biotin) to correctly folded proteins (e., GFP, antibodies, streptavidin) in site-specific manner, and with yields that surpass those of any reported using phage display technology.

TL;DR: A novel labeling strategy is applied to cholera toxin subunit A1 in the context of a pre-assembled holotoxin allowing tracking of its intracellular trafficking pathway and identification of host proteins involved in cell intoxication.

TL;DR: In this paper, a transamidase recognition sequence that allows ligating an acyl donor compound with a nucleophilic acyl acceptor in the presence of transamic acid was presented.

TL;DR: By equipping the N terminus or C terminus of the proteins of interest with a set of click handles using sortase A, followed by a strain-promoted click reaction, unnatural N- to-N and C-to-C linked (hetero) fusion proteins are established.

TL;DR: A genome-wide genetic screen in human KBM7 cells was performed to uncover host factors used by PE, several of which were confirmed by CRISPR/Cas9-gene editing in a different cell type, including GPR107, an orphan G-protein-coupled receptor.