Malini Varadarajan

TL;DR: Using insertional mutagenesis to develop a screening method to generate null alleles in a human cell line haploid for all chromosomes except chromosome 8, host factors essential for infection with influenza and genes encoding important elements of the biosynthetic pathway of diphthamide, which are required for the cytotoxic effects ofdiphtheria toxin and exotoxin A are identified.

TL;DR: The generation and characterization of iPSCs derived from human chronic myeloid leukemia cells are described and it is shown that, despite the presence of oncogenic mutations, these cells acquired pluripotency by the expression of 4 transcription factors and underwent differentiation into cell types derived of all 3 germ layers during teratoma formation.

TL;DR: This work uses a retroviral gene-trap vector to generate insertions in >98% of the genes expressed in a human cancer cell line that is haploid for all but one of its chromosomes to identify 743 mutations distributed over 12 human genes important for intoxication by four different CDTs.

TL;DR: Ploegh et al. as mentioned in this paper developed a mutagenesis-based screening approach in human cells using insertional mutagenisation in KBM7 cells, a chronic myeloid leukemia cell line that is haploid for all chromosomes except chromosome 81.

TL;DR: A critical role is uncovered for MFSD2A by acting as a putative TM transporter at the plasma membrane by identifying transmembrane helical amino acid residues essential for mediating TM sensitivity.