Carla P. Guimaraes

TL;DR: This protocol describes the use of sortase-mediated reactions to label the N terminus of any given protein of interest with a variety of probes such as fluorophores, biotin or even to other proteins.

TL;DR: This work uses a retroviral gene-trap vector to generate insertions in >98% of the genes expressed in a human cancer cell line that is haploid for all but one of its chromosomes to identify 743 mutations distributed over 12 human genes important for intoxication by four different CDTs.

TL;DR: Ploegh et al. as mentioned in this paper developed a mutagenesis-based screening approach in human cells using insertional mutagenisation in KBM7 cells, a chronic myeloid leukemia cell line that is haploid for all chromosomes except chromosome 81.

TL;DR: Stochichiometric site-specific labeling on a scale not easily achievable by genetic fusions is demonstrated and introduction of a labile dipeptide linker at the N terminus of a T-cell epitope improves proteasome-dependent class I MHC-restricted peptide cross-presentation when delivered by αDEC205 in vitro and in vivo.

TL;DR: A novel and efficient cyclization method that uses the peptidyl‐transferase activity of the Staphylococcus aureus enzyme sortase A to cyclize linear synthetic precursor peptides to provide a general method for easy and efficient manufacturing of large cyclic peptides.