Catarina Brito

TL;DR: These in vitro/ex vivo models represent a distinct move to capture the realities of tumor biology in situ, but significant characterization work still remains to be done in order to show that their biochemical circuitry accurately reflects that of a tumor.

TL;DR: The main principles for the bioprocessing of hPSCs are discussed, highlighting the impact of environmental factors, novel 3D culturing approaches and integrated bioreactor strategies for controlling hPSC culture outcome.

TL;DR: Perfusion bioreactor cultures of primary human hepatocyte spheroids maintain a liver‐specific activity and architecture and are thus suitable for drug testing in a long‐term, repeated‐dose format.

TL;DR: This position paper highlights several major gaps in understanding of phytochemical bioavailability, including effects of food processing, changes during digestion, involvement of cellular transporters in influx/efflux through the gastrointestinal epithelium,Changes during colonic fermentation, and their phase I and phase II metabolism following absorption.

TL;DR: 2D culture and 3D mono- and stromal co-culture models of increasing complexity have been established and cross-comparisons made using three standard cell carcinoma lines: MCF7, LNCaP, NCI-H1437 and the adaptable methodologies described here should guide the choice of appropriate simple and complex in vitro models.