Charlotte Kristensen

TL;DR: These de novo pathways represent the first examples of one-cell microbial generation of these valuable compounds from glucose in S. pombe yeast and baker's yeast, as well as in Saccharomyces cerevisiae, which have not previously been metabolically engineered to produce any valuable, industrially scalable, white biotech commodity.

TL;DR: Results demonstrate that insertion of high-flux pathways directing synthesis and intracellular storage of high amounts of a cyanogenic glucoside or a glucosinolate is achievable in transgenic A. thaliana plants with marginal inadvertent effects on the transcriptome and metabolome.

TL;DR: UGT85B1 has a rather broad substrate specificity in vitro but shows regiospecificity, demanding the presence of a sterically unhindered hydroxyl group e.g. as part of a cyanohydrin function, as a primary alcohol or as a phenolic hydroxy group.

TL;DR: It is demonstrated that it is possible to obtain improved catalytic properties by combining domains from both closely as well as more distantly related glycosyltransferases to gain improved substrate specificity and catalytic efficiency.

TL;DR: A substantial improvement is obtained for the dataset studied by the polynomial-hyperbolic spot shape model in combination with the Box-Cox transformation and the spatial statistical models are used to correct spot measurements with saturation by extrapolating the censored data.