Jørgen Hansen

TL;DR: These de novo pathways represent the first examples of one-cell microbial generation of these valuable compounds from glucose in S. pombe yeast and baker's yeast, as well as in Saccharomyces cerevisiae, which have not previously been metabolically engineered to produce any valuable, industrially scalable, white biotech commodity.

TL;DR: An in silico metabolic engineering strategy was designed using a set of stoichiometric modelling tools applied to the yeast genome-scale metabolic network that led to overproducing strains of vanillin producing S. cerevisiae strain.

TL;DR: Electrophoretic karyotyping analyses, restriction fragment length polymorphism maps of PCR-amplified MET2 gene fragments, and the sequence analysis of a part of the twoMET2 gene alleles found support the notion that these two strains constitute hybrids between Saccharomyces cerevisiae andSaccharomycles bayanus.

TL;DR: The data suggest that the yeast cell is able to accommodate, express and propagate genetic material that originates from different species, and the very existence of the resulting natural hybrids indicates that such hybrids are well adapted to their habitats.

TL;DR: A minimal set of three P450s in combination with a single acetyl transferase was identified that catalyzes the conversion of 13R-manoyl oxide into forskolin as demonstrated by transient expression in Nicotiana benthamiana.