C
Chikako Nishitani
Researcher at National Agriculture and Food Research Organization
Publications - 52
Citations - 1339
Chikako Nishitani is an academic researcher from National Agriculture and Food Research Organization. The author has contributed to research in topics: PEAR & Gene. The author has an hindex of 19, co-authored 48 publications receiving 1065 citations.
Papers
More filters
Journal ArticleDOI
Efficient Genome Editing in Apple Using a CRISPR/Cas9 system.
Chikako Nishitani,Narumi Hirai,Sadao Komori,Masato Wada,Kazuma Okada,Keishi Osakabe,Toshiya Yamamoto,Yuriko Osakabe +7 more
TL;DR: This study describes induction of a targeted gene mutation in the endogenous apple phytoene desaturase (PDS) gene using the CRISPR/Cas9 system, and demonstrates that genome editing can be practically applied to modify the apple genome.
Journal ArticleDOI
CRISPR-Cas9-mediated genome editing in apple and grapevine.
Yuriko Osakabe,Zhenchang Liang,Chong Ren,Chikako Nishitani,Keishi Osakabe,Masato Wada,Sadao Komori,Mickael Malnoy,Riccardo Velasco,Michele Poli,Minhee Jung,Ok Jae Koo,Roberto Viola,Chidananda Nagamangala Kanchiswamy +13 more
TL;DR: This protocol provides a stepwise protocol for the design and transfer of CRISPR–Cas9 components to apple and grapevine protoplasts, followed by verification of highly efficient targeted mutagenesis, and regeneration of plants following the plasmid-mediated delivery of components.
Journal ArticleDOI
Integrated Reference Genetic Linkage Maps of Pear Based on SSR and AFLP Markers
Toshiya Yamamoto,Tetsuya Kimura,S. Terakami,Chikako Nishitani,Yutaka Sawamura,Toshihiro Saito,Kazuo Kotobuki,Tateki Hayashi +7 more
TL;DR: These pear reference maps may enable to identify the location of genes of interest and QTLs (quantitative trait loci), and to analyze the syntenic genome structure with other Maloideae species.
Journal ArticleDOI
Identification of QTLs for fruit quality traits in Japanese apples: QTLs for early ripening are tightly related to preharvest fruit drop
Miyuki Kunihisa,Shigeki Moriya,Kazuyuki Abe,Kazuma Okada,Takashi Haji,Takeshi Hayashi,Hoytaek Kim,Chikako Nishitani,Shingo Terakami,Toshiya Yamamoto +9 more
TL;DR: This study constructed genetic linkage maps of the Japanese apple cultivars ‘Orin’ and ‘Akane’ using F1 seedlings derived from a cross between these cultivars, and found that a QTL for soluble solids content in LG16 could be explained by the amount of sorbitol and fructose.
Journal ArticleDOI
Transcriptome analysis of carnation (Dianthus caryophyllus L.) based on next-generation sequencing technology
Koji Tanase,Chikako Nishitani,Hideki Hirakawa,Sachiko Isobe,Satoshi Tabata,Akemi Ohmiya,Takashi Onozaki +6 more
TL;DR: The large EST database generated from these sequences is an informative resource for identifying genes involved in various biological processes in carnation and provides an EST resource for understanding the genetic diversity of this plant.