Chongyi Chen

TL;DR: It is shown that positive supercoiling buildup on a DNA segment by transcription slows down transcription elongation and eventually stops transcription initiation, and proves that transcriptional bursting of highly expressed genes in bacteria is primarily caused by reversible gyrase dissociation from and rebinding to aDNA segment, changing the supercoiled level of the segment.

TL;DR: Super-resolution imaging in live Escherichia coli reveals protein clusters that sequester DNA loci and organize the chromosome, and demonstrates that H-NS plays a key role in global chromosome organization in bacteria.

TL;DR: An improved single-cell WGA method, Linear Amplification via Transposon Insertion (LIANTI), is reported, which outperforms existing methods, enabling micro-CNV detection with kilobase resolution and direct observation of stochastic firing of DNA replication origins, which differs from cell to cell.

TL;DR: The design and construction of a genetic sequential logic circuit in Escherichia coli that can generate different outputs in response to the same input signal on the basis of its internal state and ‘memorize’ the output is reported.

TL;DR: This article showed that post-mitotic neurons accumulate unexpectedly high levels of DNA single-strand breaks (SSBs) at specific sites within the genome, which are repaired by PARP1 and XRCC1-dependent mechanisms.