Chris A. Helliwell

TL;DR: In this article, the authors examined design rules for efficient gene silencing, in terms of both the proportion of independent transgenic plants showing silencing and the degree of silencing.

TL;DR: A generic vector is made that allows a simple, single PCR product from a gene of interest to be easily converted into a highly effective ihpRNA silencing construct, and a high-throughput vector that should facilitate the cloning of gene libraries or large numbers of defined genes, such as those in EST collections, using an in vitro recombinase system.

TL;DR: It is shown that FLC is a component of a multimeric protein complex in vivo and that more than one FLC polypeptides can be present in the complex.

TL;DR: It is shown that the flowering repressor SVP is controlled by the autonomous, thermosensory, and gibberellin pathways, and directly represses SOC1 transcription in the shoot apex and leaf, and that the interaction between SVP and FLC mediated by various flowering genetic pathways governs the integration of flowering signals.

TL;DR: It is discussed here that RNA-induced gene silencing approaches are also likely to be effective for investigating plant gene function in a high-throughput, genome-wide manner.