Andrew P. Gleave

TL;DR: It is shown that a relatively recent (>50 million years ago) genome-wide duplication has resulted in the transition from nine ancestral chromosomes to 17 chromosomes in the Pyreae, which partly support the monophyly of the ancestral paleohexaploidy of eudicots.

TL;DR: In this article, the authors examined design rules for efficient gene silencing, in terms of both the proportion of independent transgenic plants showing silencing and the degree of silencing.

TL;DR: A generic vector is made that allows a simple, single PCR product from a gene of interest to be easily converted into a highly effective ihpRNA silencing construct, and a high-throughput vector that should facilitate the cloning of gene libraries or large numbers of defined genes, such as those in EST collections, using an in vitro recombinase system.

TL;DR: In this article, a series of plasmid vectors for transient gene expression using Agrobacterium, infiltrated into Nicotiana benthamiana leaves, are described and compared to conventional binary vectors for stable transformation such as transformation selection genes.

TL;DR: Utilisation of these vectors in Agrobacterium-mediated transformation of tobacco demonstrated efficient T-DNA transfer to the plant genome.