Christian Gieffers

TL;DR: The results suggest that vertebrate cohesins are regulated by a novel prophase pathway which is distinct from the APC pathway that controls cohesin in yeast.

TL;DR: Immunofluorescence microscopy using phospho‐antibodies indicates that APC phosphorylation is initiated in prophase during nuclear uptake of cyclin B1, implying thatAPC activation is initiated by Cdk1 already in the nuclei of late prophase cells.

TL;DR: Microinjection of antibodies against subunits of the anaphase-promoting complex/cyclosome (APC/C) or against human Cdc20 (fizzy) arrested cells at metaphase and stabilized both cyclins A and B1 suggest that the APC/ C is activated as cells enter mitosis and immediately targets cyclin A for degradation, whereas the spindle assembly checkpoint delays the degradation of cyclin B1 until the metaphase to anaphases transition.

TL;DR: It is shown that, in addition to being absent from nonproliferating cells, CDC6 is targeted for ubiquitin-mediated proteolysis by the anaphase promoting complex (APC)/cyclosome in G(1) and in quiescent cells, suggesting that this process is part of a mechanism that ensures the timely licensing of replication origins during G

TL;DR: Biochemically characterized subcomplexes of the anaphase-promoting complex (APC) of the human APC suggest that the different TPR subunits of the APC might function as receptors that interact with the C termini of regulatory proteins such as CDH1, CDC20, and APC10.