Christine A. Daigh

TL;DR: Feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material is reported.

TL;DR: In this paper, the authors describe a strategy to model blood vessel development using a well-defined induced pluripotent stem cell-derived endothelial cell type (iPSC-EC) cultured within engineered platforms that mimic the 3D microenvironment.

TL;DR: It is shown that a single matrix protein in combination with growth factors and a hypoxic environment is sufficient to generate from pluripotent cells hematopoietic progenitors capable of differentiating further in mature cell types of different lineages of the blood system.

TL;DR: In this paper, methods for automated culture of embryonic stem cells (ESCs) such as human ESCs are discussed. But these methods employ optimized culture media and limited proteolytic treatment to separate cell clusters for expansion.

TL;DR: This new flow cytometry‐based VWF:RCo assay is simple, accurate, specific and sensitive, particularly for type 2 VWD, and significantly lower than the current manual platelet aggregation assay.