T
Tenneille Ludwig
Researcher at WiCell
Publications - 41
Citations - 5407
Tenneille Ludwig is an academic researcher from WiCell. The author has contributed to research in topics: Embryonic stem cell & Stem cell. The author has an hindex of 22, co-authored 39 publications receiving 5078 citations. Previous affiliations of Tenneille Ludwig include University of Wisconsin-Madison & Wisconsin Alumni Research Foundation.
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Journal ArticleDOI
Derivation of human embryonic stem cells in defined conditions.
Tenneille Ludwig,Mark E. Levenstein,Jeffrey M. Jones,Jeffrey M. Jones,W. Travis Berggren,Erika R Mitchen,Jennifer L. Frane,Leann J Crandall,Christine A. Daigh,Kevin R. Conard,Marian S Piekarczyk,Rachel A. Llanas,James A. Thomson +12 more
TL;DR: Feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material is reported.
Journal ArticleDOI
Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells
Ren-He Xu,Ren-He Xu,Ruthann M. Peck,Dong S. Li,Xuezhu Feng,Tenneille Ludwig,James A. Thomson,James A. Thomson +7 more
TL;DR: It is demonstrated that hESCs cultured in unconditioned medium (UM) are subjected to high levels of B MP signaling activity, which is reduced in CM, and the BMP antagonist noggin synergizes with basic fibroblast growth factor (bFGF) to repress BMP signaling and sustain undifferentiated proliferation of hESC in the absence of fibroblasts or CM.
Journal ArticleDOI
Feeder-independent culture of human embryonic stem cells.
Tenneille Ludwig,Tenneille Ludwig,Veit Bergendahl,Mark E. Levenstein,Junying Yu,Mitchell D. Probasco,James A. Thomson +6 more
TL;DR: Modifications to the medium (mTeSR1) that include the use of animal-sourced proteins (bovine serum albumin (BSA) and Matrigel) and cloned zebrafish basic fibroblast growth factor (zbFGF) are described.
Journal ArticleDOI
Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage
Katherine Amps,Peter W. Andrews,George Anyfantis,Lyle Armstrong,Stuart Avery,Hossein Baharvand,Julie C. Baker,Duncan Baker,Maria D. Barbadillo Muñoz,Stephen J. Beil,Nissim Benvenisty,Dalit Ben-Yosef,Juan Carlos Biancotti,Alexis Bosman,Romulo M. Brena,Daniel R. Brison,Gunilla Caisander,Marãa V. Camarasa,Jieming Chen,Eric Chiao,Young Min Choi,Andre Choo,D.M. Collins,Alan Colman,Jeremy M. Crook,George Q. Daley,Anne Dalton,Paul A. De Sousa,Chris Denning,J.M. Downie,Petr Dvorak,Karen Dyer Montgomery,Anis Feki,Angela Ford,Victoria Fox,Ana Maria Fraga,Tzvia Frumkin,Lin Ge,Paul J. Gokhale,Tamar Golan-Lev,Hamid Gourabi,Michal Gropp,Lu GuangXiu,Aleš Hampl,Katie Harron,Lyn Healy,Wishva Herath,Frida Holm,Outi Hovatta,Johan Hyllner,Maneesha S. Inamdar,Astrid K. Irwanto,Tetsuya Ishii,Marisa Jaconi,Ying Jin,Susan J. Kimber,Sergey Kiselev,Barbara B. Knowles,Oded Kopper,Valeri Kukharenko,Anver Kuliev,Maria A. Lagarkova,Peter W. Laird,Majlinda Lako,Andrew L. Laslett,Neta Lavon,Dong Ryul Lee,Jeoung Eun Lee,Chunliang Li,Linda S. Lim,Tenneille Ludwig,Yu Ma,Edna Maltby,Ileana Mateizel,Yoav Mayshar,Maria Mileikovsky,Stephen L. Minger,Takamichi Miyazaki,Shin Yong Moon,Harry Moore,Christine L. Mummery,Andras Nagy,Norio Nakatsuji,Kavita Narwani,Steve Oh,Sun Kyung Oh,Cia Olson,Timo Otonkoski,Fei Pan,In-Hyun Park,Steve Pells,Martin F. Pera,Lygia da Veiga Pereira,Ouyang Qi,Grace Selva Raj,Benjamin Reubinoff,Alan Robins,Paul Robson,Janet Rossant,Ghasem Hosseini Salekdeh,Thomas C. Schulz,Karen Sermon,Jameelah Sheik Mohamed,Hui Shen,Eric S Sherrer,Kuldip S. Sidhu,Shirani Sivarajah,Heli Skottman,Claudia Spits,Glyn Stacey,Raimund Strehl,Nick Strelchenko,Hirofumi Suemori,Bowen Sun,Riitta Suuronen,Kazutoshi Takahashi,Timo Tuuri,Parvathy Venu,Yuri Verlinsky,Dorien Ward-van Oostwaard,Daniel J. Weisenberger,Yue Wu,Shinya Yamanaka,Lorraine E. Young,Qi Zhou +124 more
TL;DR: Of these genes, BCL2L1 is a strong candidate for driving culture adaptation of ES cells, and single-nucleotide polymorphism analysis revealed that they included representatives of most major ethnic groups.
Journal ArticleDOI
Basic fibroblast growth factor support of human embryonic stem cell self-renewal.
Mark E. Levenstein,Tenneille Ludwig,Ren-He Xu,Rachel A. Llanas,Kaitlyn VanDenHeuvel‐Kramer,Daisy Manning,James A. Thomson +6 more
TL;DR: Comparing the ability of unconditioned medium (UM) supplemented with 4, 24, 40, 80, 100, and 250 ng/ml FGF2 to sustain low‐density human ES cell cultures through multiple passages suggests that fibroblasts and fibroblast‐conditioned medium sustain human ES cells in part by stabilizing FGF signaling above a critical threshold.