Journal ArticleDOI
Derivation of human embryonic stem cells in defined conditions.
Tenneille Ludwig,Mark E. Levenstein,Jeffrey M. Jones,Jeffrey M. Jones,W. Travis Berggren,Erika R Mitchen,Jennifer L. Frane,Leann J Crandall,Christine A. Daigh,Kevin R. Conard,Marian S Piekarczyk,Rachel A. Llanas,James A. Thomson +12 more
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TLDR
Feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material is reported.Abstract:
We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.read more
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Human DNA methylomes at base resolution show widespread epigenomic differences
Ryan Lister,Mattia Pelizzola,Robert H. Dowen,R. David Hawkins,Gary C. Hon,Julian Tonti-Filippini,Joseph R. Nery,Leonard Lee,Zhen Ye,Que Minh Ngo,Lee Edsall,Jessica Antosiewicz-Bourget,Jessica Antosiewicz-Bourget,Ron Stewart,Ron Stewart,Victor Ruotti,Victor Ruotti,A. Harvey Millar,James A. Thomson,Bing Ren,Bing Ren,Joseph R. Ecker +21 more
TL;DR: The first genome-wide, single-base-resolution maps of methylated cytosines in a mammalian genome, from both human embryonic stem cells and fetal fibroblasts, along with comparative analysis of messenger RNA and small RNA components of the transcriptome, several histone modifications, and sites of DNA-protein interaction for several key regulatory factors were presented in this article.
Journal ArticleDOI
Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro
Jason R. Spence,Christopher N. Mayhew,Scott A. Rankin,Matthew F. Kuhar,Jefferson E. Vallance,Kathryn Tolle,Elizabeth E. Hoskins,Vladimir V. Kalinichenko,Susanne I. Wells,Aaron M. Zorn,Noah F. Shroyer,James M. Wells +11 more
TL;DR: A robust and efficient process is established to direct the differentiation of human PSCs into intestinal tissue in vitro using a temporal series of growth factor manipulations to mimic embryonic intestinal development and indicates that human intestinal stem cells form de novo during development.
Journal ArticleDOI
Hotspots of aberrant epigenomic reprogramming in human induced pluripotent stem cells
Ryan Lister,Mattia Pelizzola,Yasuyuki S. Kida,R. David Hawkins,Joseph R. Nery,Gary C. Hon,Jessica Antosiewicz-Bourget,Jessica Antosiewicz-Bourget,Ronan C. O'Malley,Rosa Castanon,Sarit Klugman,Michael Downes,Ruth T. Yu,Ron Stewart,Ron Stewart,Bing Ren,Bing Ren,James A. Thomson,Ronald M. Evans,Joseph R. Ecker +19 more
TL;DR: The first whole-genome profiles of DNA methylation at single-base resolution in five human iPSC lines, along with methylomes of ES cells, somatic cells, and differentiated iPSCs and ES cells are reported.
Journal ArticleDOI
Embryonic stem cells.
Helen J. Rippon,Anne E. Bishop +1 more
TL;DR: This work reviews the history of murine and human ES cell Lines, including practical and ethical aspects of ES cell isolation from pre‐implantation embryos, maintenance of undifferentiated ES cell lines in the cell culture environment, and differentiation of ES cells in vitro and in vivo into mature somatic cell types.
Journal ArticleDOI
Distinct epigenomic landscapes of pluripotent and lineage-committed human cells
R. David Hawkins,Gary C. Hon,Leonard Lee,Que-Minh Ngo,Ryan Lister,Mattia Pelizzola,Lee Edsall,Samantha Kuan,Ying Luu,Sarit Klugman,Jessica Antosiewicz-Bourget,Zhen Ye,Celso A. Espinoza,Saurabh Agarwahl,Li Shen,Victor Ruotti,Victor Ruotti,Wei Wang,Ron Stewart,Ron Stewart,James A. Thomson,Joseph R. Ecker,Bing Ren,Bing Ren +23 more
TL;DR: By comparing the chromatin-modification profiles and DNA methylomes in hESCs and primary fibroblasts, it is found that nearly one-third of the genome differs in chromatin structure.
References
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor
Noboru Sato,Laurent Meijer,Laurent Meijer,Leandros Skaltsounis,Paul Greengard,Ali H. Brivanlou +5 more
TL;DR: It is shown that activation of the canonical Wnt pathway is sufficient to maintain self-renewal of both HESCs and MESCs, and the use of GSK-3-specific inhibitors such as BIO may have practical applications in regenerative medicine.
Journal ArticleDOI
BMP Induction of Id Proteins Suppresses Differentiation and Sustains Embryonic Stem Cell Self-Renewal in Collaboration with STAT3
TL;DR: It is reported that bone morphogenetic proteins (BMPs) act in combination with LIF to sustain self-renewal and preserve multilineage differentiation, chimera colonization, and germline transmission properties.
Journal ArticleDOI
Clonally Derived Human Embryonic Stem Cell Lines Maintain Pluripotency and Proliferative Potential for Prolonged Periods of Culture
Michal Amit,Melissa K. Carpenter,Margaret S. Inokuma,Choy-Pik Chiu,Charles P. Harris,Michelle A. Waknitz,Joseph Itskovitz-Eldor,James A. Thomson +7 more
TL;DR: The clonal derivation of two human ES cell lines, H9.1 and H.2, demonstrates the pluripotency of single human ES cells, the maintenance of pluripOTency during an extended period of culture, and the long-term self-renewing properties of cultured human ES Cells.
Journal ArticleDOI
BMP4 initiates human embryonic stem cell differentiation to trophoblast.
Ren-He Xu,Xin Chen,Dong S. Li,Rui Li,Gregory C. Addicks,Clay Glennon,Thomas P. Zwaka,James A. Thomson +7 more
TL;DR: Bone morphogenetic protein 4 (BMP4), a member of the transforming growth factor-beta (TGF-beta) superfamily, induces the differentiation of human ES cells to trophoblast.