Christof Hepp

TL;DR: The force-dependent kinetics of DNA uptake are in remarkable agreement with a translocation ratchet model, whereby the periplasmic ComE protein acts as a chaperone that rectifies DNA diffusion through the outer membrane by reversible binding, indicating that ComE is directly involved in the uptake process.

TL;DR: It is concluded that the periplasm can act as a considerable reservoir for imported DNA with ComE governing the amount of DNA stored potentially for transport through the inner membrane.

TL;DR: A methodology for virus detection and identification that uses a convolutional neural network to distinguish between microscopy images of single intact particles of different viruses, which achieves labeling, imaging and virus identification in less than five minutes and does not require any lysis, purification or amplification steps.

TL;DR: In this paper, the authors introduced a rapid fluorescence in situ hybridization (FISH) protocol capable of detecting influenza virus, avian infectious bronchitis virus and SARS-CoV-2 specifically and quantitatively in approximately 20min, in virus cultures, combined nasal and throat swabs with added virus and likely patient samples.

TL;DR: It is proposed that these three secretion systems employ different molecular implementations of the translocation ratchet mechanism, which may include DNA import by the type II secretion/type IV pilus system, DNA export by thetype IV secretion system, and protein exportBy the type I secretion system.